Showing 1–25 of 31 documents
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Sharja Phuljhele, Pratima Beck, Ashish Sinha, Anil Kumar Saroj
…reas for extra-pulmonary TB, CBNAAT (Gene-Xpert) and liquid-culture are available choices. Objectives: To establish the comparative diagnostic efficacy of CBNAAT and Liquid-culture in clinically presumptive childhood Tuberculosis. Outcome: Assess sensitivity and specificity of CB…
Clémentine Maureau, Laurence Parmeland, Nathalie Rousseau, Matthieu Pecquet
…reus and its resistance to methicillin as soon as the blood culture is positive. We evaluate performance of GeneXpert MRSA/SA Blood Culture assay (Cepheid®) before and after interpretation of the positivity levels of the various probes estimated by the Cycle threshold (Ct), as we…
Sobia Rasheed, Salma aziz, Irfan Mehmud, Tanzeela Rani, Nur Ul Ain Umair, et al.
…te than sputum, but not routinely applied. OBJECTIVE: Using culture as gold standard, compare the diagnostic precision of GeneXpert and Smear microscopy for the diagnosis of tuberculosis in Paediatric patients. This cross-sectional validation study included 250 children and invol…
Randall P, Esmail A, Wilson L, Makambwa E, Pooran A, et al.
…h diagnostic accuracy. However, DNA-based diagnostic tests (GeneXpert Ultra), more sensitive than an earlier versions, have recently become available. Given that the diagnosis of TBP is challenging, we performed a comparative diagnostic accuracy study comparing both assays. Metho…
Sharma K, Sharma M, Sharma A, Dhillon MS
…(TruPlus), a chip-based portable machine, was compared with GeneXpert Ultra (GxUltra) for diagnosing drug-resistant OATB. Design 115 synovial fluid and pus specimens [22 culture-positive confirmed, 58 culture-negative clinically-suspected, 35 non-TB controls] processed between 20…
Liu R, Liang Q, Shang Y, Ma Y, Wang F, et al.
…from digestive tract. We aimed to assess the performance of GeneXpert on stool and gastric lavage fluid (GALF) in diagnosing TB among patients with severe pulmonary TB. Methods We enrolled adults with suspected pulmonary TB who were unable to produce sputum at visit between Janua…
E. A. Lapteva, I. V. Kovalenko, O. I. Burakevich, Minsk Regional Tuberculosis Dispensary, Minsk region, Belarus, Olga Kharevich, et al.
GeneXpert MTB/Rif assay for rapid diagnosis of tuberculosis and detection of rifampicin resistance can reduce the time to diagnosis and initiation of treatment. Aim. To evaluate the sensitivity and specificity of the GeneXpert MTB/Rif assay compared with traditional methods of de…
Ai JW, Zhou X, Xu T, Yang M, Chen Y, et al.
…. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the…
Jingwen Ai, Xian Zhou, Teng Xu, Minling Yang, Yuanyuan Chen, et al.
…. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the…
Jingwen Ai, Xian Zhou, Teng Xu, Minling Yang, Yuanyuan Chen, et al.
…. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the…
Heather J. Zar, Mark P. Nicol
…inical or radiologic signs, paucibacillary disease, and low sensitivity of microbiologic diagnosis.2 However, microbiologic confirmation is needed for accurately defining the burden of disease, effective treatment (particularly for drug-resistant tuberculosis), and limiting unnec…
Sahar Mourad, Ayman Ibrahim Baess, Reham E. Abdullah, Nadia M. Ragab
…e Xpert in sputum samples of PTB-suspect patients regarding sensitivity, specificity, timing to positivity, and drug susceptibility compared with the gold standard Löwenstein–Jensen (LJ) mycobacterial culture. Patients and methods This is a retrospective clinical study. Records o…
Ministry of Health, Saudi Arabia
…: Open-label study. Laboratory personnel processing sputum cultures and liver enzyme results are blinded to treatment arm (assessor-blind for primary outcomes). The DSMB will conduct unblinded interim analyses. SAMPLE SIZE: Total 200 participants (100 per arm). Based on 80% sp…
Ma J, Wang L, Zheng X, Cui H, Sha W
…ronchoalveolar lavage fluid was collected for mycobacterial culture and acid-fast staining, GeneXpert MTB/RIF, and DGPMC assay. The diagnostic performance of DGPMC assay was evaluated against the composite reference standard. Results Overall, 240 patients were included in this tr…
Ying Gao, Zhongfeng Huang, Xiaoting Jing, Silian Chen, Qiuyue Chen
…ethods demonstrating significant limitations including poor sensitivity and prolonged diagnostic timeframes, particularly problematic for smear-negative tuberculosis cases. Targeted next-generation sequencing (tNGS) combined with flexible bronchoscopy represents an innovative dia…
Buchera FS, Silago V, Japhet G, Mtemisika CI, Damiano P, et al.
…uberculosis (TB) at selected hospitals in Mwanza, Tanzania. GeneXpert and conventional bacteriological culture and sensitivity were used for the detection of TB and other pathogenic bacteria, respectively. STATA version 13.0 was used for data analysis. The median (IQR) age of par…
Chang Y, Zhang M, Liu G, Wu X, Yan Q, et al.
…tudy showed that TB nestMIRA performed slightly better than GeneXpert MTB/RIF (Xpert) (85.27% vs. 82.17%) and significantly better than culture (55.81%) and acid-fast bacillus (AFB) smear (38.76%). The TB nestMIRA assay offers speed, specificity, sensitivity, and convenience. We …
J. Mira Gutiérrez, LaShaunda L. Malone, John Mukisa, Ezekiel Mupere, Catherine M. Stein, et al.
…st X-ray findings, and microbiologic testing (AFB smear and culture; GeneXpert Ultra) of two induced sputum samples. Counts (percentages), means [+/- standard deviation], or median [quartiles]. χ 2: Chi-squared test. MWU: Mann–Whitney U test. QFT: QuantiFERON-TB Gold. * Statistic…
Mareen Esmail Hassan, Noor Salman Kadhim Al-Khafaji, Azhar Omran Lateef Althahab
…(AFB) smear microscopy (20.6% detection), Löwenstein–Jensen culture, and the GeneXpert MTB/RIF assay (23.3% detection). Serum levels of miR-361-5p and miR-3125 were measured by RT-qPCR and normalized using the 2^−ΔΔCt method. Both miRNAs were significant level upregulated in TB p…
Melanie Etti, Amédine Duret, Che Cootauco, Padmasayee Papineni
…icular sites (48.8% vs. 10.7%, p < 0.001). Forty cases were culture-positive with 38 fully-sensitive, 1 MDR and 1 isoniazid-resistant. Twenty-nine patients had positive tissue or pus GeneXpert MTB/RIF®. A 42% of patients were treated empirically on a clinico-radiological basis. M…
Cao J, Gu Y, Wu XC, Cheng LP, Wang L, et al.
…eolar lavage fluid (BALF) with acid-fast staining, MGIT 960 culture, pathological examination, and Xpert for isolated pulmonary nodules caused by MTB infection were compared using receiver operating characteristic (ROC) analysis. Results There were 135 patients, 64 with isolated …
Soheila Shaghaghian, Narsis Ghahramani, Erfan Sadeghi, Zahra Zarei, Sina Nasrollahian, et al.
…here is controversy regarding the indications of conducting GeneXpert assay for patients suspected of tuberculosis (TB). Therefore, we compared the accuracy of the assay between patients with and without positive results of acid-fast bacilli (AFB) smear and HIV. **METHOD:** The …
Deepak Kumar, Shivang Sharma, Gopal Krishana Bohra, Mahendra Kumar Garg, Naresh Kumar Midha, et al.
…ond line) the month prior were excluded. With molecular and culture methods, best practices were done to find opportunistic infections, including tuberculosis. Results A total of 60 patients were enrolled, and urinary LAM was positive in 18 (30%) cases. Among culture or GeneXpert…
Evarist Chiweka, Thomas Maroa, Hosiana Temba, Joseph Ponera, Sharifa Athumani, et al.
…ithout active PTB. The diagnosis of PTB was performed using GeneXpert MTB/RIF assay and culture. Blood was collected from cases and controls for measuring CRP levels during recruitment. We compared socio-demographic characteristics, clinical and laboratory parameters obtained dur…
Evarist Chiweka, Thomas Maroa, Hosiana Temba, Joseph Ponera, Sharifa Athumani, et al.
…ithout active PTB. The diagnosis of PTB was performed using GeneXpert MTB/RIF assay and culture. Blood was collected from cases and controls for measuring CRP levels during recruitment. We compared socio-demographic characteristics, clinical and laboratory parameters obtained dur…