Showing 1–25 of 53 documents
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Gao M, Wu Q, Wang X, Sun X, Li M, et al.
…73 cases of PTB and 42 cases of EPTB. Sputum smears, sputum culture, TB-RNA, GeneXpert, and urine LAM results were obtained from all patients before treatment. Using the composite reference standard as the reference, the diagnostic performance of these methods for PTB and EPTB wa…
Sharja Phuljhele, Pratima Beck, Ashish Sinha, Anil Kumar Saroj
…reas for extra-pulmonary TB, CBNAAT (Gene-Xpert) and liquid-culture are available choices. Objectives: To establish the comparative diagnostic efficacy of CBNAAT and Liquid-culture in clinically presumptive childhood Tuberculosis. Outcome: Assess sensitivity and specificity of CB…
Clémentine Maureau, Laurence Parmeland, Nathalie Rousseau, Matthieu Pecquet
…reus and its resistance to methicillin as soon as the blood culture is positive. We evaluate performance of GeneXpert MRSA/SA Blood Culture assay (Cepheid®) before and after interpretation of the positivity levels of the various probes estimated by the Cycle threshold (Ct), as we…
Sobia Rasheed, Salma aziz, Irfan Mehmud, Tanzeela Rani, Nur Ul Ain Umair, et al.
…te than sputum, but not routinely applied. OBJECTIVE: Using culture as gold standard, compare the diagnostic precision of GeneXpert and Smear microscopy for the diagnosis of tuberculosis in Paediatric patients. This cross-sectional validation study included 250 children and invol…
Rushna Wazahat, Mehvash Haider, Debasish Chattopadhya, Mridu Dudeja
…ected to staining (Ziehl-Neelson and fluorescent staining), culture, GeneXpert, and line probe assay. Results: The sensitivity, specificity, and strength of association, i.e., kappa (k) value of LED-FM, ZN, and GeneXpert, were calculated using standard formulae using solid cultur…
Randall P, Esmail A, Wilson L, Makambwa E, Pooran A, et al.
…h diagnostic accuracy. However, DNA-based diagnostic tests (GeneXpert Ultra), more sensitive than an earlier versions, have recently become available. Given that the diagnosis of TBP is challenging, we performed a comparative diagnostic accuracy study comparing both assays. Metho…
Chen P, Sun W, He Y
…ds In this study, we collected the results of both mNGS and culture of 70 specimens from suspected TB patients at the Shanghai Pulmonary Hospital of Tongji University. Results of GeneXpert MTB/RIF assay (Xpert) were obtained from 19 patients. We also assessed the diagnostic perfo…
Tummidi Santosh, Prashant Sood, Ashok Rattan, Deepa Dave, Puneeta Nagiredla
…assessed the diagnostic and quantitative performance of the GeneXpert Mycobacterium tuberculosis/resistance to rifampicin (MTB/RIF) assay on pulmonary and extrapulmonary samples processed in an Indian laboratory. We examined this to assess if GeneXpert can complement existing dia…
Sharma K, Sharma M, Sharma A, Dhillon MS
…(TruPlus), a chip-based portable machine, was compared with GeneXpert Ultra (GxUltra) for diagnosing drug-resistant OATB. Design 115 synovial fluid and pus specimens [22 culture-positive confirmed, 58 culture-negative clinically-suspected, 35 non-TB controls] processed between 20…
Liu R, Liang Q, Shang Y, Ma Y, Wang F, et al.
…from digestive tract. We aimed to assess the performance of GeneXpert on stool and gastric lavage fluid (GALF) in diagnosing TB among patients with severe pulmonary TB. Methods We enrolled adults with suspected pulmonary TB who were unable to produce sputum at visit between Janua…
Krishnakumariamma K, Ellappan K, Muthuraj M, Tamilarasu K, Kumar SV, et al.
…c health concern worldwide. We evaluated the performance of GeneXpert MTB/RIF (GeneXpert) for the diagnosis of TBM. In addition, genetic diversity and drug susceptibility profiling of Mtb strains isolated from TBM patients were also investigated. A total of 293 TBM suspected cere…
Xu JC, Shi X, Ma X, Gu WF, Fang ZX, et al.
… (OR 2.22, 95% CI 1.17-4.28, p = 0.014). In biopsy samples, GeneXpert (Xpert) showed higher sensitivity (96.6% [91.6-98.7], p 95%) and specificity (nearly 100%) of any methods. In non-biopsy samples, only T-SPOT. TB (T-SPOT) showed higher sensitivity than culture (90.9% [62.3-99.…
E. A. Lapteva, I. V. Kovalenko, O. I. Burakevich, Minsk Regional Tuberculosis Dispensary, Minsk region, Belarus, Olga Kharevich, et al.
GeneXpert MTB/Rif assay for rapid diagnosis of tuberculosis and detection of rifampicin resistance can reduce the time to diagnosis and initiation of treatment. Aim. To evaluate the sensitivity and specificity of the GeneXpert MTB/Rif assay compared with traditional methods of de…
Jingwen Ai, Xian Zhou, Teng Xu, Minling Yang, Yuanyuan Chen, et al.
…. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the…
Jingwen Ai, Xian Zhou, Teng Xu, Minling Yang, Yuanyuan Chen, et al.
…. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the…
Ai JW, Zhou X, Xu T, Yang M, Chen Y, et al.
…. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the…
Mukesh Rathod, Abdul Qavi, Vineeta Mittal, Ajai Kumar Singh, Pradeep Kumar Maurya, et al.
…he smear testing of the cerebrospinal fluid (CSF) has a low sensitivity and cultures, though having higher sensitivity, take a long time in TBM diagnosis. The present study was done to evaluate the proportion of positive Gene Xpert test, a rapid nucleic acid amplification test, i…
Heather J. Zar, Mark P. Nicol
…inical or radiologic signs, paucibacillary disease, and low sensitivity of microbiologic diagnosis.2 However, microbiologic confirmation is needed for accurately defining the burden of disease, effective treatment (particularly for drug-resistant tuberculosis), and limiting unnec…
Sahar Mourad, Ayman Ibrahim Baess, Reham E. Abdullah, Nadia M. Ragab
…e Xpert in sputum samples of PTB-suspect patients regarding sensitivity, specificity, timing to positivity, and drug susceptibility compared with the gold standard Löwenstein–Jensen (LJ) mycobacterial culture. Patients and methods This is a retrospective clinical study. Records o…
Ministry of Health, Saudi Arabia
…: Open-label study. Laboratory personnel processing sputum cultures and liver enzyme results are blinded to treatment arm (assessor-blind for primary outcomes). The DSMB will conduct unblinded interim analyses. SAMPLE SIZE: Total 200 participants (100 per arm). Based on 80% sp…
Ma J, Wang L, Zheng X, Cui H, Sha W
…ronchoalveolar lavage fluid was collected for mycobacterial culture and acid-fast staining, GeneXpert MTB/RIF, and DGPMC assay. The diagnostic performance of DGPMC assay was evaluated against the composite reference standard. Results Overall, 240 patients were included in this tr…
Ferroudja Yamouni, Fatma Zohra Henniche, Malika Ifticene, Madjid Chabani, Doria Bensersa, et al.
…s of pulmonary and extra-pulmonary tuberculosis compared to culture. 2) To compare the rifampicin results resistance obtained by GX with the phenotypic sensitivity test. Materials and methods: Retrospective study carried out over a period of five years, from May 2017 to June 2022…
Ying Gao, Zhongfeng Huang, Xiaoting Jing, Silian Chen, Qiuyue Chen
…ethods demonstrating significant limitations including poor sensitivity and prolonged diagnostic timeframes, particularly problematic for smear-negative tuberculosis cases. Targeted next-generation sequencing (tNGS) combined with flexible bronchoscopy represents an innovative dia…
Edgar Ortíz‐Brizuela, Dick Menzies
…been temporary, and could have simply reflected the greater sensitivity of the tests used in many studies compared with those used in routine services (eg, culture or GeneXpert vs acid-fast bacillus smear). The harms resulting from false positive results were not assessed in this…
Buchera FS, Silago V, Japhet G, Mtemisika CI, Damiano P, et al.
…uberculosis (TB) at selected hospitals in Mwanza, Tanzania. GeneXpert and conventional bacteriological culture and sensitivity were used for the detection of TB and other pathogenic bacteria, respectively. STATA version 13.0 was used for data analysis. The median (IQR) age of par…