Showing 26–50 of 53 documents
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Grønningen E, Nanyaro M, Sviland L, Ngadaya E, Muller W, et al.
…istry based MPT64 antigen detection test has shown improved sensitivity compared to current laboratory tests in the resource limited diagnostic setting. The aim of this study was to validate the implementability and diagnostic performance of the test in PLWHIV and HIV negative ad…
National Heart and Lung Institute
…quencing (WGS) on positive Mycobacterium tuberculosis (MTb) culture samples. Given the lack of research and large global ASM workforce, 1 million of whom are in Tanzania, the results of this study will assist in the development and introduction of interventions to reduce the ris…
Chang Y, Zhang M, Liu G, Wu X, Yan Q, et al.
…tudy showed that TB nestMIRA performed slightly better than GeneXpert MTB/RIF (Xpert) (85.27% vs. 82.17%) and significantly better than culture (55.81%) and acid-fast bacillus (AFB) smear (38.76%). The TB nestMIRA assay offers speed, specificity, sensitivity, and convenience. We …
J. Mira Gutiérrez, LaShaunda L. Malone, John Mukisa, Ezekiel Mupere, Catherine M. Stein, et al.
…st X-ray findings, and microbiologic testing (AFB smear and culture; GeneXpert Ultra) of two induced sputum samples. Counts (percentages), means [+/- standard deviation], or median [quartiles]. χ 2: Chi-squared test. MWU: Mann–Whitney U test. QFT: QuantiFERON-TB Gold. * Statistic…
Mareen Esmail Hassan, Noor Salman Kadhim Al-Khafaji, Azhar Omran Lateef Althahab
…(AFB) smear microscopy (20.6% detection), Löwenstein–Jensen culture, and the GeneXpert MTB/RIF assay (23.3% detection). Serum levels of miR-361-5p and miR-3125 were measured by RT-qPCR and normalized using the 2^−ΔΔCt method. Both miRNAs were significant level upregulated in TB p…
Raunak Bir, Urvashi B Singh, Hitender Gautam, Nishant Verma, Kiran Bala, et al.
…entional diagnostic methods, such as smear microscopy, lack sensitivity and timeliness. This study evaluates the prognostic value of time to positivity (TTP) from liquid culture and cycle threshold (CT) values from GeneXpert/rifampicin (Cepheid, Sunnyvale, CA) in predicting treat…
Melanie Etti, Amédine Duret, Che Cootauco, Padmasayee Papineni
…icular sites (48.8% vs. 10.7%, p < 0.001). Forty cases were culture-positive with 38 fully-sensitive, 1 MDR and 1 isoniazid-resistant. Twenty-nine patients had positive tissue or pus GeneXpert MTB/RIF®. A 42% of patients were treated empirically on a clinico-radiological basis. M…
Keertan Dheda, MD/PhD
…rug resistant sequencing pipelines for diagnostic accuracy, sensitivity, specificity and predictive value as compared to culture based phenotypic drug susceptibility testing. Aim 3: To compare the feasibility, accuracy, turn-aroundtime, and cost implications of the above-mention…
Adrian Martineau, PhD
…tic individuals associates with the ability to visualize or culture bacilli in blood, which has been done in patients with active TB. Moreover, our existing method for detection of Mtb DNA in CD34+ PBMC using dPCR is not currently suitable for field use, as samples require length…
Cao J, Gu Y, Wu XC, Cheng LP, Wang L, et al.
…eolar lavage fluid (BALF) with acid-fast staining, MGIT 960 culture, pathological examination, and Xpert for isolated pulmonary nodules caused by MTB infection were compared using receiver operating characteristic (ROC) analysis. Results There were 135 patients, 64 with isolated …
Ebrahim Variava, Tumelo Moloantoa, Neil Martinson
…a remarkable leap forward for tuberculosis diagnostics. The GeneXpert tuberculosis assay (Cepheid, Sunnyvale, CA, USA) provides a result in 2 h, is both highly sensitive and specific, and is a major improvement over conventional smear microscopy. Moreover, simultaneous testing fo…
Soheila Shaghaghian, Narsis Ghahramani, Erfan Sadeghi, Zahra Zarei, Sina Nasrollahian, et al.
…here is controversy regarding the indications of conducting GeneXpert assay for patients suspected of tuberculosis (TB). Therefore, we compared the accuracy of the assay between patients with and without positive results of acid-fast bacilli (AFB) smear and HIV. **METHOD:** The …
Uddin MKM, Islam A, Jabin MS, Alam T, Khair S, et al.
…ared to QuantiFERON-TB Gold Plus (QFT-Plus) for all groups. GeneXpert, culture, and microscopy were used to confirm TB microbiologically. Results TBE had an overall agreement of 85.9% (95% CI, 82.5% to 88.7%), positive percent agreement of 86.1% (95% CI, 80.6% to 90.5%), and nega…
Aarhus University Hospital
…m sampling (faecal and a buccal/tongue swab and saliva) for GeneXpert Ultra PCR (Xpert) as state-of-the-art add-ons to the routine diagnostic pathway for TB. This adds to the key components of the WHO "End TB" strategy - early diagnosis - and if successful, may be rapidly approve…
Deepak Kumar, Shivang Sharma, Gopal Krishana Bohra, Mahendra Kumar Garg, Naresh Kumar Midha, et al.
…ond line) the month prior were excluded. With molecular and culture methods, best practices were done to find opportunistic infections, including tuberculosis. Results A total of 60 patients were enrolled, and urinary LAM was positive in 18 (30%) cases. Among culture or GeneXpert…
Evarist Chiweka, Thomas Maroa, Hosiana Temba, Joseph Ponera, Sharifa Athumani, et al.
…ithout active PTB. The diagnosis of PTB was performed using GeneXpert MTB/RIF assay and culture. Blood was collected from cases and controls for measuring CRP levels during recruitment. We compared socio-demographic characteristics, clinical and laboratory parameters obtained dur…
Evarist Chiweka, Thomas Maroa, Hosiana Temba, Joseph Ponera, Sharifa Athumani, et al.
…ithout active PTB. The diagnosis of PTB was performed using GeneXpert MTB/RIF assay and culture. Blood was collected from cases and controls for measuring CRP levels during recruitment. We compared socio-demographic characteristics, clinical and laboratory parameters obtained dur…
Evarist Chiweka
…ithout active PTB. The diagnosis of PTB was performed using GeneXpert MTB/RIF assay and culture. Blood was collected from cases and controls for measuring CRP levels during recruitment. We compared socio-demographic characteristics, clinical and laboratory parameters obtained dur…
Hoa NB, Fajans M, Nguyen Van H, Vu Ngoc B, Nguyen Viet N, et al.
…ompared to a microbiological reference standard (MRS) using GeneXpert Ultra and TB culture testing. Estimated uLAM concentrations above plate specific calculated limit of detection (LOD) were considered uLAM positive. Additional microbiological testing was conducted for possible …
H Sekharamahanti, D Nerusu, K Chintharala, L Dankwa
…monary tuberculosis (PTB) but negative initial spot CBNAAT (GeneXpert) results present a common diagnostic dilemma. Simple, low-cost sampling approaches such as induced sputum or 24-hour pooled sputum may enhance diagnostic yield before bronchoscopy. However, comparative data usi…
Hnin Aung, K Balasundaram, John A. Goss, Hemal H. Patel, Prositosth Haldar, et al.
…ural fluid poses challenges in the diagnosis of pleural TB. GeneXpert (GX) is a molecular test which detects the presence of MTB DNA and is validated in sputum specimens. However, its role in pleural TB is unclear. We sought to analyse how a diagnosis of pleural TB may be achieve…
Seth B, Bernardo J, Sulis C
… infection isolation (AII). The US FDA approved the Cepheid GeneXpert-MTB-Rif® to support removing patients from AII. The FDA requires that “either one or two” separate sputum specimens be examined. To clarify this statement, the National Tuberculosis Controllers’ Association and…
Grønningen E, Nanyaro M, Sviland L, Ngadaya E, Muller W, et al.
…istry based MPT64 antigen detection test has shown improved sensitivity compared to current laboratory tests. The aim of this study was to implement and validate the test performance in a resource limited African setting. Presumptive pediatric (0-18 y) EPTB patients were prospect…
Bahizi G, Majwala RK, Kisaka S, Nyombi A, Musisi K, et al.
…istance), presents challenges to TB control. In Uganda, the GeneXpert test provides point-of-care testing for TB and rifampicin resistance. Patients identified with RR-TB receive culture-based drug susceptibility testing (DST) to identify additional resistance, if any. There are …
Chaisson LH, Duong D, Cattamanchi A, Roemer M, Handley MA, et al.
…mplementation of a sputum molecular testing algorithm using GeneXpert MTB/RIF (Xpert; Cepheid) to guide discontinuation of isolation. Main outcomes and measures We measured the proportion of patients with molecular testing ordered and completed; the accuracy of the molecular test…