Showing 26–47 of 47 documents
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Chang Y, Zhang M, Liu G, Wu X, Yan Q, et al.
…tudy showed that TB nestMIRA performed slightly better than GeneXpert MTB/RIF (Xpert) (85.27% vs. 82.17%) and significantly better than culture (55.81%) and acid-fast bacillus (AFB) smear (38.76%). The TB nestMIRA assay offers speed, specificity, sensitivity, and convenience. We …
J. Mira Gutiérrez, LaShaunda L. Malone, John Mukisa, Ezekiel Mupere, Catherine M. Stein, et al.
…st X-ray findings, and microbiologic testing (AFB smear and culture; GeneXpert Ultra) of two induced sputum samples. Counts (percentages), means [+/- standard deviation], or median [quartiles]. χ 2: Chi-squared test. MWU: Mann–Whitney U test. QFT: QuantiFERON-TB Gold. * Statistic…
Mareen Esmail Hassan, Noor Salman Kadhim Al-Khafaji, Azhar Omran Lateef Althahab
…(AFB) smear microscopy (20.6% detection), Löwenstein–Jensen culture, and the GeneXpert MTB/RIF assay (23.3% detection). Serum levels of miR-361-5p and miR-3125 were measured by RT-qPCR and normalized using the 2^−ΔΔCt method. Both miRNAs were significant level upregulated in TB p…
Raunak Bir, Urvashi B Singh, Hitender Gautam, Nishant Verma, Kiran Bala, et al.
…entional diagnostic methods, such as smear microscopy, lack sensitivity and timeliness. This study evaluates the prognostic value of time to positivity (TTP) from liquid culture and cycle threshold (CT) values from GeneXpert/rifampicin (Cepheid, Sunnyvale, CA) in predicting treat…
Melanie Etti, Amédine Duret, Che Cootauco, Padmasayee Papineni
…icular sites (48.8% vs. 10.7%, p < 0.001). Forty cases were culture-positive with 38 fully-sensitive, 1 MDR and 1 isoniazid-resistant. Twenty-nine patients had positive tissue or pus GeneXpert MTB/RIF®. A 42% of patients were treated empirically on a clinico-radiological basis. M…
Cao J, Gu Y, Wu XC, Cheng LP, Wang L, et al.
…eolar lavage fluid (BALF) with acid-fast staining, MGIT 960 culture, pathological examination, and Xpert for isolated pulmonary nodules caused by MTB infection were compared using receiver operating characteristic (ROC) analysis. Results There were 135 patients, 64 with isolated …
Ebrahim Variava, Tumelo Moloantoa, Neil Martinson
…a remarkable leap forward for tuberculosis diagnostics. The GeneXpert tuberculosis assay (Cepheid, Sunnyvale, CA, USA) provides a result in 2 h, is both highly sensitive and specific, and is a major improvement over conventional smear microscopy. Moreover, simultaneous testing fo…
Soheila Shaghaghian, Narsis Ghahramani, Erfan Sadeghi, Zahra Zarei, Sina Nasrollahian, et al.
…here is controversy regarding the indications of conducting GeneXpert assay for patients suspected of tuberculosis (TB). Therefore, we compared the accuracy of the assay between patients with and without positive results of acid-fast bacilli (AFB) smear and HIV. **METHOD:** The …
Uddin MKM, Islam A, Jabin MS, Alam T, Khair S, et al.
…ared to QuantiFERON-TB Gold Plus (QFT-Plus) for all groups. GeneXpert, culture, and microscopy were used to confirm TB microbiologically. Results TBE had an overall agreement of 85.9% (95% CI, 82.5% to 88.7%), positive percent agreement of 86.1% (95% CI, 80.6% to 90.5%), and nega…
Deepak Kumar, Shivang Sharma, Gopal Krishana Bohra, Mahendra Kumar Garg, Naresh Kumar Midha, et al.
…ond line) the month prior were excluded. With molecular and culture methods, best practices were done to find opportunistic infections, including tuberculosis. Results A total of 60 patients were enrolled, and urinary LAM was positive in 18 (30%) cases. Among culture or GeneXpert…
Evarist Chiweka, Thomas Maroa, Hosiana Temba, Joseph Ponera, Sharifa Athumani, et al.
…ithout active PTB. The diagnosis of PTB was performed using GeneXpert MTB/RIF assay and culture. Blood was collected from cases and controls for measuring CRP levels during recruitment. We compared socio-demographic characteristics, clinical and laboratory parameters obtained dur…
Evarist Chiweka, Thomas Maroa, Hosiana Temba, Joseph Ponera, Sharifa Athumani, et al.
…ithout active PTB. The diagnosis of PTB was performed using GeneXpert MTB/RIF assay and culture. Blood was collected from cases and controls for measuring CRP levels during recruitment. We compared socio-demographic characteristics, clinical and laboratory parameters obtained dur…
Hoa NB, Fajans M, Nguyen Van H, Vu Ngoc B, Nguyen Viet N, et al.
…ompared to a microbiological reference standard (MRS) using GeneXpert Ultra and TB culture testing. Estimated uLAM concentrations above plate specific calculated limit of detection (LOD) were considered uLAM positive. Additional microbiological testing was conducted for possible …
H Sekharamahanti, D Nerusu, K Chintharala, L Dankwa
…monary tuberculosis (PTB) but negative initial spot CBNAAT (GeneXpert) results present a common diagnostic dilemma. Simple, low-cost sampling approaches such as induced sputum or 24-hour pooled sputum may enhance diagnostic yield before bronchoscopy. However, comparative data usi…
Hnin Aung, K Balasundaram, John A. Goss, Hemal H. Patel, Prositosth Haldar, et al.
…ural fluid poses challenges in the diagnosis of pleural TB. GeneXpert (GX) is a molecular test which detects the presence of MTB DNA and is validated in sputum specimens. However, its role in pleural TB is unclear. We sought to analyse how a diagnosis of pleural TB may be achieve…
Seth B, Bernardo J, Sulis C
… infection isolation (AII). The US FDA approved the Cepheid GeneXpert-MTB-Rif® to support removing patients from AII. The FDA requires that “either one or two” separate sputum specimens be examined. To clarify this statement, the National Tuberculosis Controllers’ Association and…
Grønningen E, Nanyaro M, Sviland L, Ngadaya E, Muller W, et al.
…istry based MPT64 antigen detection test has shown improved sensitivity compared to current laboratory tests. The aim of this study was to implement and validate the test performance in a resource limited African setting. Presumptive pediatric (0-18 y) EPTB patients were prospect…
Bahizi G, Majwala RK, Kisaka S, Nyombi A, Musisi K, et al.
…istance), presents challenges to TB control. In Uganda, the GeneXpert test provides point-of-care testing for TB and rifampicin resistance. Patients identified with RR-TB receive culture-based drug susceptibility testing (DST) to identify additional resistance, if any. There are …
Chaisson LH, Duong D, Cattamanchi A, Roemer M, Handley MA, et al.
…mplementation of a sputum molecular testing algorithm using GeneXpert MTB/RIF (Xpert; Cepheid) to guide discontinuation of isolation. Main outcomes and measures We measured the proportion of patients with molecular testing ordered and completed; the accuracy of the molecular test…
Emelia Konadu Danso, Prince Asare, Amanda Yaa Tetteh, Phillip Tetteh, Augustine Asare Boadu, et al.
…nt outcomes. We conducted a prospective cohort study of 758 GeneXpert-confirmed pulmonary TB patients, of whom 75 had DM. Demographic, clinical, radiographic, and anthropometric data were collected at baseline. Sputum samples were cultured for mycobacterial isolation, and the obt…
Ershova JV, Volchenkov GV, Somova TR, Kuznetsova TA, Kaunetis NV, et al.
Background The main advantage of GeneXpert MTB/RIF® (Xpert) molecular diagnostic technology is the rapid detection of M.tuberculosis DNA and mutations associated with rifampicin (RIF) resistance for timely initiation of appropriate treatment and, consequently, preventing further …
Hickey AJ, Cummings MJ, Zafari Z, Louh IK, Li J, et al.
…berculosis (MTB) to reduce risk of nosocomial transmission. GeneXpert MTB/RIF (Xpert) is more rapid and accurate than sputum smear microscopy, but it is not routinely used to 'rule out' infectious pulmonary TB among hospitalized patients in the United States. We sought to evaluat…