Decreased Methylenetetrahydrofolate Reductase Activity Leads to Increased Sensitivity to <i>para</i> -Aminosalicylic Acid in Mycobacterium tuberculosis
Ji-fang Yu, Jin-tian Xu, Shan-shan Yang, Mei-na Gao, Hao-rui Si, Dong-yan Xiong, Jing Gu, Zhi-long Wu, et al. (10 authors)
Antimicrobial Agents and Chemotherapy · 2021-11
Abstract
Tuberculosis (TB), caused by Mycobacterium tuberculosis , is one of the most fatal diseases in the world. Methylenetetrahydrofolate reductase (MTHFR) catalyzes the production of 5-methyltetrahydrofolate (5-CH 3 -THF), which is required for the de novo biosynthesis of methionine in bacteria. Here, we identified Rv2172c as an MTHFR in M. tuberculosis through in vitro and in vivo analyses and determined that the protein is essential for the in vitro growth of the bacterium.
MeSH terms
- Mycobacterium tuberculosis
- Tuberculosis
- Methylenetetrahydrofolate reductase
- Methionine
- Mutant
- Enzyme
- Reductase
- Mutation
- Biology
- Microbiology
- Biochemistry
- Chemistry
- Biosynthesis
- Antifolate
- Dihydrofolate reductase
- Antibiotics