Transcriptomic dataset of <i>Mycolicibacterium smegmatis</i> exposed to an imidazo[1,2-<i>b</i>][1,2,4,5]tetrazine

Deciphering the mechanism of action of novel anti-tuberculosis compounds is a key step in the drug development process. We have previously described a number of imidazo[1,2- b ][1,2,4,5]tetrazines with a promising activity on Mycobacterium tuberculosis [1]. These compounds had predicted activity as serine‑threonine protein kinase inhibitors, however spontaneous drug resistant Mycolicibacterium smegmatis mc 2 155 (formerly Mycobacterium smegmatis ) revealed only the mycobacterial mechanism of resistance to imidazo[1,2- b ][1,2,4,5]tetrazines: mutations in MSMEG_1380 gene lead to overexpression of the mmpS5-mmpL5 operon in M. smegmatis , thus providing resistance to imidazo[1,2- b ][1,2,4,5]tetrazines via enhanced efflux [2]. Here we report the RNA sequencing data of M. smegmatis mc 2 155 culture treated with one of the imidazo[1,2- b ][1,2,4,5]tetrazines for 1.5 h and the untreated culture as a control. The mapped reads showed that a total of 1386 genes are differentially expressed in this experiment. A further analysis of these data can shed light of the mechanism of action of imidazo[1,2- b ][1,2,4,5]tetrazines. The data generated by RNA-seq (raw reads) have been deposited to NCBI sequence read archive (SRA) and have been assigned a BioProject accession number PRJNA615922.