Showing 76–100 of 200 documents
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Sun Y, Gao L, Xia H, Yang Z, Deng S, et al.
OBJECTIVE: To evaluate the accuracy of molecular diagnostics for the detection of drug-resistant tuberculosis (TB) in Chinese patients. METHOD: Seven databases were searched for eligible studies that evaluated the accuracy of molecular diagnostics against drug susceptibility test…
Jean de Dieu Iragena, Achilles Katamba, Anandi Martin, Moses Joloba, Willy Ssengooba
**BACKGROUND:** Rapid tuberculosis (TB) diagnostics are essential for TB control. Factors influencing the uptake of these technologies in Africa are not well-documented for all technologies and are completely undocumented for some countries. We conducted a survey to collect the s…
Marya Getchell, John Pastor Ansah, Dodge Lim, Ramon Basilio, Francis Tablizo, et al.
**BACKGROUND:** New and effective tools for detecting drug-resistant tuberculosis (DR-TB) include GeneXpert XDR and targeted Next Generation Sequencing (tNGS). However, data on their implementation in high TB-burden settings is limited. We aimed to determine cost-effectiveness of…
Assiut University
* Detection of sensitivity and specificity of microscopic examination and Molecular studies using polymerase chain reaction (PCR) Mycobacterium tuberculosis (MTB)/ non tuberculous mycobacterium (NTM) MDR-TB compared to culture which is the gold standard technique in the diagnosis…
Ajbani K, Kazi M, Agrawal U, Jatale R, Soman R, et al.
Background We evaluated the performance of pyrosequencing, a genotypic test which detects TB and XDR-defining mutations within 6 h, directly on CSF samples for diagnosing TB meningitis(TBM). Methods This retrospective, diagnostic accuracy study was conducted in Hinduja hospital, …
Maurer FP, Shubladze N, Kalmambetova G, Felker I, Kuchukhidze G, et al.
The World Health Organization (WHO) recently revised its guidelines for rapid diagnosis of drug-resistant tuberculosis (TB). This study aimed to investigate if TB reference diagnostic services are prepared to support these revisions. An online survey was performed among 44 TB Nat…
Sanjay Singh Negi, Priyanka Singh, Sachin Chandraka, Ujjwala Gaikwad, Padma Das, et al.
Introduction: Effective management of Extrapulmonary Tuberculosis (EPTB) requires simultaneous identification of Mycobacterium tuberculosis (M.tuberculosis) complex, NonTuberculous Mycobacteria (NTM) and Rifampicin (RIF) sensitivity pattern for early and effective evidence based …
World Health Organization
This web annex to the WHO operational handbook on tuberculosis, Module 3: Diagnosis, provides technical guidance for culture-based drug susceptibility testing (DST) of anti-tuberculosis drugs used in the treatment of tuberculosis. The manual describes standardized phenotypic DST …
Kabasakalyan E, Davtyan K, Cholakyans V, Mirzoyan A, Kentenyants K, et al.
Introduction In 2013, the National Tuberculosis (TB) Program of Armenia introduced GeneXpert MTB/RIF (Xpert) assay to address World Health Organization (WHO) target of 80% (2020) of notified new and relapse TB cases to be tested with WHO recommended rapid diagnostic methods. This…
Alagna R, Combary A, Tagliani E, Sawadogo LT, Saouadogo T, et al.
Backgrounds The laboratory plays a critical role in tuberculosis (TB) control by providing testing for diagnosis, treatment monitoring, and surveillance at each level of the health care system. Weak accessibility to TB diagnosric services still represents a big concern in many li…
Shun-Chung Hsueh, Chieh-Lung Chen, Chang-Ru Lin, Hsiu-Hsien Lin, Tai-Fen Lee, et al.
**OBJECTIVES:** To evaluate the diagnostic performance of Roche cobas MTB and MTB-RIF/INH assays for detecting Mycobacterium tuberculosis complex (MTBC) and resistance to isoniazid (INH) and rifampicin (RIF). **METHODS:** This multicentre study was conducted in Taiwan between Se…
Tuelo Mogashoa, Justice T Ngom, Johannes Loubser, Kedumetse Seru, Tuduetso Molefi, et al.
**BACKGROUND:** Undetected rifampicin resistance is a threat to global tuberculosis (TB) control efforts by delaying effective treatment. In different studies, non-canonical rpoB mutations outside the rifampicin resistance-determining region have been reported at varying prevalen…
Rajendran P, Padmapriyadarsini C, Nair S, Sivakumar S
…agnostic landscape. According to the WHO 2021 Guidelines on Diagnostics, the NAATs used in TB diagnosis at the point of care (POC) include Xpert MTB/RIF a cartridge-based test manufactured by Cepheid, and Truenat a chip-based test manufactured by Molbio. Other POC tests that are …
Scott K Heysell, MD
Major Research Aim: To study novel molecular diagnostics and the pharmacokinetic variability among a spectrum of TB disease states, including severe forms of TB like disseminated TB, TB meningitis and drug resistant TB, among adults and children from multiple international sites.…
Mendelsohn SC, Fiore-Gartland A, Penn-Nicholson A, Mulenga H, Mbandi SK, et al.
Background A rapid, blood-based triage test that allows targeted investigation for tuberculosis at the point of care could shorten the time to tuberculosis treatment and reduce mortality. We aimed to test the performance of a host blood transcriptomic signature (RISK11) in diagno…
Thijs Hoffman, Maurice Mwansa, Angel Mubanga, John Kondwelani Mateyo
…a very low bacillary load in the initial sample. Additional diagnostics for rifampicin resistance were available in 56% of patients, and revealed rifampicin susceptibility in 28% of tested patients. Rifampicin-susceptible TB was found more often if the bacillary load in the initi…
Shi J, Tao B, Li Z, Song H, Wu J, et al.
Objective Drug-resistant tuberculosis (DR-TB) is a growing problem worldwide. The rapid drug susceptibility test (DST) of DR-TB enables the timely administration of a chemotherapy regimen that effectively treats DR-TB. GeneChip has been reported as a novel molecular diagnostic to…
Kamolwat P, Nonghanphithak D, Chaiprasert A, Smithtikarn S, Pungrassami P, et al.
BACKGROUND: Whole-genome sequencing (WGS) is a promising tool for the detection of drug-resistant TB (DR-TB). To date, there have been few comparisons of diagnostic performance of WGS and phenotypic drug susceptibility testing (DST) in DR-TB. METHODS: We compared drug resistance-…
World Health Organization
This consolidated WHO guideline provides evidence-based recommendations on rapid diagnostic technologies for the detection of tuberculosis (TB) and drug-resistant TB. Developed within the framework of the WHO End TB Strategy and global commitments adopted at the 2018 United Natio…
Siti Soidah, Toto Subroto, Irvan Faizal, Muhammad Yusuf
Rifampicin-resistant tuberculosis (RR-TB) remains a major threat to global TB control, primarily driven by mutations in thegene of(Mtb). Most resistance-conferring mutations occur within the 81-base pair RIF resistance determining region (RRDR), particularly at codons S450L, H445…
Hadizadeh Tasbiti A, Yari S, Siadat SD, Karimipoor M, Badmasti F, et al.
Tuberculosis (TB) is a sizable public health threat in the world. This study was conducted to determine the differential protein composition between susceptible and MDRTB strains. Tuberculosis proteins were extracted by Triton™ X-114 and ammonium sulfate. Two-dimensional gel elec…
Vipin Deo Tiwari, Mazher Maqusood, G. Ramakrishna, Rajul Rastogi
Background: Compare the diagnostic sensitivity of ZN staining and CBNAAT using Mycobacterium Culture as a gold standard in BAL fluid among sputum smear negative or non- sputum producing patients with suspected pulmonary tuberculosis.Subjects and Methods:Our prospective observatio…
Saeed Khan, Zia Ullah, Lala Ruhk, Muhammad Haroon, Afsheen Bashir, et al.
Background: Tuberculosis (TB) is considered to be one of the most prominent reasons for death globally caused by a single microorganism. However with the advancement in the field of human health, researchers have mostly focused on the strategies to eradicate the TB but it has not…
Patel J, Upadhyay M, Kundnani V, Merchant Z, Jain S, et al.
Study design A cross-sectional observational study. Objective This study aims to determine the diagnostic accuracy, sensitivity, and specificity of the Xpert MTB/RIF assay (Mycobacterium Tuberculosis/Rifampicin resistance) for the detection of spinal Tuberculosis (TB) and rifampi…
Noori Lee, Hojeong Kwon, Yoohyun Hwang, Sungweon Ryoo
Abstract Background: This study aims to investigate the usefulness of GeneXpert MTB/RIF (Xpert) in diagnosing rifampicin (RIF) resistance among the patients in the secluded sanatoria of North Korea. This article is a follow up of a report that explored the characteristics of drug…