Showing 1–24 of 24 documents
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Gao M, Wu Q, Wang X, Sun X, Li M, et al.
…73 cases of PTB and 42 cases of EPTB. Sputum smears, sputum culture, TB-RNA, GeneXpert, and urine LAM results were obtained from all patients before treatment. Using the composite reference standard as the reference, the diagnostic performance of these methods for PTB and EPTB wa…
Randall P, Esmail A, Wilson L, Makambwa E, Pooran A, et al.
…h diagnostic accuracy. However, DNA-based diagnostic tests (GeneXpert Ultra), more sensitive than an earlier versions, have recently become available. Given that the diagnosis of TBP is challenging, we performed a comparative diagnostic accuracy study comparing both assays. Metho…
Chen P, Sun W, He Y
…ds In this study, we collected the results of both mNGS and culture of 70 specimens from suspected TB patients at the Shanghai Pulmonary Hospital of Tongji University. Results of GeneXpert MTB/RIF assay (Xpert) were obtained from 19 patients. We also assessed the diagnostic perfo…
Sharma K, Sharma M, Sharma A, Dhillon MS
…(TruPlus), a chip-based portable machine, was compared with GeneXpert Ultra (GxUltra) for diagnosing drug-resistant OATB. Design 115 synovial fluid and pus specimens [22 culture-positive confirmed, 58 culture-negative clinically-suspected, 35 non-TB controls] processed between 20…
Liu R, Liang Q, Shang Y, Ma Y, Wang F, et al.
…from digestive tract. We aimed to assess the performance of GeneXpert on stool and gastric lavage fluid (GALF) in diagnosing TB among patients with severe pulmonary TB. Methods We enrolled adults with suspected pulmonary TB who were unable to produce sputum at visit between Janua…
Krishnakumariamma K, Ellappan K, Muthuraj M, Tamilarasu K, Kumar SV, et al.
…c health concern worldwide. We evaluated the performance of GeneXpert MTB/RIF (GeneXpert) for the diagnosis of TBM. In addition, genetic diversity and drug susceptibility profiling of Mtb strains isolated from TBM patients were also investigated. A total of 293 TBM suspected cere…
Xu JC, Shi X, Ma X, Gu WF, Fang ZX, et al.
… (OR 2.22, 95% CI 1.17-4.28, p = 0.014). In biopsy samples, GeneXpert (Xpert) showed higher sensitivity (96.6% [91.6-98.7], p 95%) and specificity (nearly 100%) of any methods. In non-biopsy samples, only T-SPOT. TB (T-SPOT) showed higher sensitivity than culture (90.9% [62.3-99.…
Ai JW, Zhou X, Xu T, Yang M, Chen Y, et al.
…. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the…
Ma J, Wang L, Zheng X, Cui H, Sha W
…ronchoalveolar lavage fluid was collected for mycobacterial culture and acid-fast staining, GeneXpert MTB/RIF, and DGPMC assay. The diagnostic performance of DGPMC assay was evaluated against the composite reference standard. Results Overall, 240 patients were included in this tr…
Buchera FS, Silago V, Japhet G, Mtemisika CI, Damiano P, et al.
…uberculosis (TB) at selected hospitals in Mwanza, Tanzania. GeneXpert and conventional bacteriological culture and sensitivity were used for the detection of TB and other pathogenic bacteria, respectively. STATA version 13.0 was used for data analysis. The median (IQR) age of par…
Grønningen E, Nanyaro M, Sviland L, Ngadaya E, Muller W, et al.
…istry based MPT64 antigen detection test has shown improved sensitivity compared to current laboratory tests in the resource limited diagnostic setting. The aim of this study was to validate the implementability and diagnostic performance of the test in PLWHIV and HIV negative ad…
Chang Y, Zhang M, Liu G, Wu X, Yan Q, et al.
…tudy showed that TB nestMIRA performed slightly better than GeneXpert MTB/RIF (Xpert) (85.27% vs. 82.17%) and significantly better than culture (55.81%) and acid-fast bacillus (AFB) smear (38.76%). The TB nestMIRA assay offers speed, specificity, sensitivity, and convenience. We …
Raunak Bir, Urvashi B Singh, Hitender Gautam, Nishant Verma, Kiran Bala, et al.
…entional diagnostic methods, such as smear microscopy, lack sensitivity and timeliness. This study evaluates the prognostic value of time to positivity (TTP) from liquid culture and cycle threshold (CT) values from GeneXpert/rifampicin (Cepheid, Sunnyvale, CA) in predicting treat…
Cao J, Gu Y, Wu XC, Cheng LP, Wang L, et al.
…eolar lavage fluid (BALF) with acid-fast staining, MGIT 960 culture, pathological examination, and Xpert for isolated pulmonary nodules caused by MTB infection were compared using receiver operating characteristic (ROC) analysis. Results There were 135 patients, 64 with isolated …
Soheila Shaghaghian, Narsis Ghahramani, Erfan Sadeghi, Zahra Zarei, Sina Nasrollahian, et al.
…here is controversy regarding the indications of conducting GeneXpert assay for patients suspected of tuberculosis (TB). Therefore, we compared the accuracy of the assay between patients with and without positive results of acid-fast bacilli (AFB) smear and HIV. **METHOD:** The …
Uddin MKM, Islam A, Jabin MS, Alam T, Khair S, et al.
…ared to QuantiFERON-TB Gold Plus (QFT-Plus) for all groups. GeneXpert, culture, and microscopy were used to confirm TB microbiologically. Results TBE had an overall agreement of 85.9% (95% CI, 82.5% to 88.7%), positive percent agreement of 86.1% (95% CI, 80.6% to 90.5%), and nega…
Hoa NB, Fajans M, Nguyen Van H, Vu Ngoc B, Nguyen Viet N, et al.
…ompared to a microbiological reference standard (MRS) using GeneXpert Ultra and TB culture testing. Estimated uLAM concentrations above plate specific calculated limit of detection (LOD) were considered uLAM positive. Additional microbiological testing was conducted for possible …
Seth B, Bernardo J, Sulis C
… infection isolation (AII). The US FDA approved the Cepheid GeneXpert-MTB-Rif® to support removing patients from AII. The FDA requires that “either one or two” separate sputum specimens be examined. To clarify this statement, the National Tuberculosis Controllers’ Association and…
Grønningen E, Nanyaro M, Sviland L, Ngadaya E, Muller W, et al.
…istry based MPT64 antigen detection test has shown improved sensitivity compared to current laboratory tests. The aim of this study was to implement and validate the test performance in a resource limited African setting. Presumptive pediatric (0-18 y) EPTB patients were prospect…
Bahizi G, Majwala RK, Kisaka S, Nyombi A, Musisi K, et al.
…istance), presents challenges to TB control. In Uganda, the GeneXpert test provides point-of-care testing for TB and rifampicin resistance. Patients identified with RR-TB receive culture-based drug susceptibility testing (DST) to identify additional resistance, if any. There are …
Chaisson LH, Duong D, Cattamanchi A, Roemer M, Handley MA, et al.
…mplementation of a sputum molecular testing algorithm using GeneXpert MTB/RIF (Xpert; Cepheid) to guide discontinuation of isolation. Main outcomes and measures We measured the proportion of patients with molecular testing ordered and completed; the accuracy of the molecular test…
Emelia Konadu Danso, Prince Asare, Amanda Yaa Tetteh, Phillip Tetteh, Augustine Asare Boadu, et al.
…nt outcomes. We conducted a prospective cohort study of 758 GeneXpert-confirmed pulmonary TB patients, of whom 75 had DM. Demographic, clinical, radiographic, and anthropometric data were collected at baseline. Sputum samples were cultured for mycobacterial isolation, and the obt…
Ershova JV, Volchenkov GV, Somova TR, Kuznetsova TA, Kaunetis NV, et al.
Background The main advantage of GeneXpert MTB/RIF® (Xpert) molecular diagnostic technology is the rapid detection of M.tuberculosis DNA and mutations associated with rifampicin (RIF) resistance for timely initiation of appropriate treatment and, consequently, preventing further …
Hickey AJ, Cummings MJ, Zafari Z, Louh IK, Li J, et al.
…berculosis (MTB) to reduce risk of nosocomial transmission. GeneXpert MTB/RIF (Xpert) is more rapid and accurate than sputum smear microscopy, but it is not routinely used to 'rule out' infectious pulmonary TB among hospitalized patients in the United States. We sought to evaluat…