Showing 1–23 of 23 documents
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Sharja Phuljhele, Pratima Beck, Ashish Sinha, Anil Kumar Saroj
…reas for extra-pulmonary TB, CBNAAT (Gene-Xpert) and liquid-culture are available choices. Objectives: To establish the comparative diagnostic efficacy of CBNAAT and Liquid-culture in clinically presumptive childhood Tuberculosis. Outcome: Assess sensitivity and specificity of CB…
Clémentine Maureau, Laurence Parmeland, Nathalie Rousseau, Matthieu Pecquet
…reus and its resistance to methicillin as soon as the blood culture is positive. We evaluate performance of GeneXpert MRSA/SA Blood Culture assay (Cepheid®) before and after interpretation of the positivity levels of the various probes estimated by the Cycle threshold (Ct), as we…
Sobia Rasheed, Salma aziz, Irfan Mehmud, Tanzeela Rani, Nur Ul Ain Umair, et al.
…te than sputum, but not routinely applied. OBJECTIVE: Using culture as gold standard, compare the diagnostic precision of GeneXpert and Smear microscopy for the diagnosis of tuberculosis in Paediatric patients. This cross-sectional validation study included 250 children and invol…
Rushna Wazahat, Mehvash Haider, Debasish Chattopadhya, Mridu Dudeja
…ected to staining (Ziehl-Neelson and fluorescent staining), culture, GeneXpert, and line probe assay. Results: The sensitivity, specificity, and strength of association, i.e., kappa (k) value of LED-FM, ZN, and GeneXpert, were calculated using standard formulae using solid cultur…
Tummidi Santosh, Prashant Sood, Ashok Rattan, Deepa Dave, Puneeta Nagiredla
…assessed the diagnostic and quantitative performance of the GeneXpert Mycobacterium tuberculosis/resistance to rifampicin (MTB/RIF) assay on pulmonary and extrapulmonary samples processed in an Indian laboratory. We examined this to assess if GeneXpert can complement existing dia…
E. A. Lapteva, I. V. Kovalenko, O. I. Burakevich, Minsk Regional Tuberculosis Dispensary, Minsk region, Belarus, Olga Kharevich, et al.
GeneXpert MTB/Rif assay for rapid diagnosis of tuberculosis and detection of rifampicin resistance can reduce the time to diagnosis and initiation of treatment. Aim. To evaluate the sensitivity and specificity of the GeneXpert MTB/Rif assay compared with traditional methods of de…
Jingwen Ai, Xian Zhou, Teng Xu, Minling Yang, Yuanyuan Chen, et al.
…. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the…
Jingwen Ai, Xian Zhou, Teng Xu, Minling Yang, Yuanyuan Chen, et al.
…. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the…
Mukesh Rathod, Abdul Qavi, Vineeta Mittal, Ajai Kumar Singh, Pradeep Kumar Maurya, et al.
…he smear testing of the cerebrospinal fluid (CSF) has a low sensitivity and cultures, though having higher sensitivity, take a long time in TBM diagnosis. The present study was done to evaluate the proportion of positive Gene Xpert test, a rapid nucleic acid amplification test, i…
Heather J. Zar, Mark P. Nicol
…inical or radiologic signs, paucibacillary disease, and low sensitivity of microbiologic diagnosis.2 However, microbiologic confirmation is needed for accurately defining the burden of disease, effective treatment (particularly for drug-resistant tuberculosis), and limiting unnec…
Sahar Mourad, Ayman Ibrahim Baess, Reham E. Abdullah, Nadia M. Ragab
…e Xpert in sputum samples of PTB-suspect patients regarding sensitivity, specificity, timing to positivity, and drug susceptibility compared with the gold standard Löwenstein–Jensen (LJ) mycobacterial culture. Patients and methods This is a retrospective clinical study. Records o…
Ferroudja Yamouni, Fatma Zohra Henniche, Malika Ifticene, Madjid Chabani, Doria Bensersa, et al.
…s of pulmonary and extra-pulmonary tuberculosis compared to culture. 2) To compare the rifampicin results resistance obtained by GX with the phenotypic sensitivity test. Materials and methods: Retrospective study carried out over a period of five years, from May 2017 to June 2022…
Ying Gao, Zhongfeng Huang, Xiaoting Jing, Silian Chen, Qiuyue Chen
…ethods demonstrating significant limitations including poor sensitivity and prolonged diagnostic timeframes, particularly problematic for smear-negative tuberculosis cases. Targeted next-generation sequencing (tNGS) combined with flexible bronchoscopy represents an innovative dia…
Edgar Ortíz‐Brizuela, Dick Menzies
…been temporary, and could have simply reflected the greater sensitivity of the tests used in many studies compared with those used in routine services (eg, culture or GeneXpert vs acid-fast bacillus smear). The harms resulting from false positive results were not assessed in this…
J. Mira Gutiérrez, LaShaunda L. Malone, John Mukisa, Ezekiel Mupere, Catherine M. Stein, et al.
…st X-ray findings, and microbiologic testing (AFB smear and culture; GeneXpert Ultra) of two induced sputum samples. Counts (percentages), means [+/- standard deviation], or median [quartiles]. χ 2: Chi-squared test. MWU: Mann–Whitney U test. QFT: QuantiFERON-TB Gold. * Statistic…
Mareen Esmail Hassan, Noor Salman Kadhim Al-Khafaji, Azhar Omran Lateef Althahab
…(AFB) smear microscopy (20.6% detection), Löwenstein–Jensen culture, and the GeneXpert MTB/RIF assay (23.3% detection). Serum levels of miR-361-5p and miR-3125 were measured by RT-qPCR and normalized using the 2^−ΔΔCt method. Both miRNAs were significant level upregulated in TB p…
Melanie Etti, Amédine Duret, Che Cootauco, Padmasayee Papineni
…icular sites (48.8% vs. 10.7%, p < 0.001). Forty cases were culture-positive with 38 fully-sensitive, 1 MDR and 1 isoniazid-resistant. Twenty-nine patients had positive tissue or pus GeneXpert MTB/RIF®. A 42% of patients were treated empirically on a clinico-radiological basis. M…
Ebrahim Variava, Tumelo Moloantoa, Neil Martinson
…a remarkable leap forward for tuberculosis diagnostics. The GeneXpert tuberculosis assay (Cepheid, Sunnyvale, CA, USA) provides a result in 2 h, is both highly sensitive and specific, and is a major improvement over conventional smear microscopy. Moreover, simultaneous testing fo…
Deepak Kumar, Shivang Sharma, Gopal Krishana Bohra, Mahendra Kumar Garg, Naresh Kumar Midha, et al.
…ond line) the month prior were excluded. With molecular and culture methods, best practices were done to find opportunistic infections, including tuberculosis. Results A total of 60 patients were enrolled, and urinary LAM was positive in 18 (30%) cases. Among culture or GeneXpert…
Evarist Chiweka, Thomas Maroa, Hosiana Temba, Joseph Ponera, Sharifa Athumani, et al.
…ithout active PTB. The diagnosis of PTB was performed using GeneXpert MTB/RIF assay and culture. Blood was collected from cases and controls for measuring CRP levels during recruitment. We compared socio-demographic characteristics, clinical and laboratory parameters obtained dur…
Evarist Chiweka, Thomas Maroa, Hosiana Temba, Joseph Ponera, Sharifa Athumani, et al.
…ithout active PTB. The diagnosis of PTB was performed using GeneXpert MTB/RIF assay and culture. Blood was collected from cases and controls for measuring CRP levels during recruitment. We compared socio-demographic characteristics, clinical and laboratory parameters obtained dur…
H Sekharamahanti, D Nerusu, K Chintharala, L Dankwa
…monary tuberculosis (PTB) but negative initial spot CBNAAT (GeneXpert) results present a common diagnostic dilemma. Simple, low-cost sampling approaches such as induced sputum or 24-hour pooled sputum may enhance diagnostic yield before bronchoscopy. However, comparative data usi…
Hnin Aung, K Balasundaram, John A. Goss, Hemal H. Patel, Prositosth Haldar, et al.
…ural fluid poses challenges in the diagnosis of pleural TB. GeneXpert (GX) is a molecular test which detects the presence of MTB DNA and is validated in sputum specimens. However, its role in pleural TB is unclear. We sought to analyse how a diagnosis of pleural TB may be achieve…