Proteomics reveals coordinated stress adaptation by a MazF toxin to conserve carbon, sustain central metabolism, and preserve PDIM biosynthesis in.

In response to host-generated stresses,(Mtb) reprograms its physiology in myriad ways to establish and maintain an infection, yet the signals that underlie this transformation are not well defined. The abundant toxin-antitoxin (TA) systems harbored in the Mtb genome, including 11 in thefamily, are thought to act as stress sensors, yet their roles are largely unknown. Although TA systems from other bacteria are generally thought to impart reversible growth arrest in response to stress, the exquisite specificity of Mtb tRNase toxins instead portends a more nuanced role. Here, we used a proteomics approach to trackprotein synthesis to uncover molecular events initiated by the Mtb MazF-mt9 toxin (MazF7, Rv2063A). First, we documented striking enrichment of enzymes and transporters derived from the contiguous 36-gene region for phthiocerol dimycocerosate (PDIM) synthesis without an accompanying increase in PDIM lipid production. This paradox was reconciled by concomitant downregulation of proteins comprising the Mce1 transporter (imports host fatty acids), cholesterol breakdown, and β-oxidation enzymes (limiting the PDIM precursor methylmalonyl-CoA). Thus, increased catalytic efficiency of the PDIM pathway appears to offset substrate starvation to ensure adequate production of PDIMs essential for Mtb early immune escape and virulence. Finally, isocitrate lyase 1 levels also increased, which in this context are expected to primarily catalyze the glyoxylate shunt to sustain central carbon metabolism while minimizing carbon loss. These exacting proteomic signatures are paralleled within the bedaquiline-treated Mtb transcriptome, highlighting a critical role for MazF-mt9 in orchestrating Mtb stress survival.IMPORTANCEThe bacterial pathogen that causes tuberculosis,(Mtb), must survive a gauntlet of immune assaults to establish an infection. Here, we determined that in response to host-imposed stresses, this pathogen enlists the action of a tRNase, the MazF-mt9 toxin, to reprogram the translatome and orchestrate metabolic remodeling to ensure adequate production of specialized phthiocerol dimycocerosate (PDIM) lipids on the cell surface, which contribute to early immune evasion. This toxin also upregulates isocitrate lyase 1 as a complementary survival-oriented adaptation that conserves carbon and sustains central metabolism for essential cellular functions. Thus, this toxin-mediated cooperative reprogramming toward preservation of PDIMs and central metabolism under lipid precursor-limiting conditions likely enables Mtb to successfully infect and survive in the host lung. Overall, the MazF-mt9-mediated protein expression signatures align with the transcriptome signatures of Mtb cells during bedaquiline treatment, suggesting a precise and essential role for this toxin in Mtb stress survival.