Design of New Primer Sets for the Development of a Loop-Mediated Isothermal Amplification for Rapid Detection of.

is a pathogenic bacterial agent that causes meningococcal meningitis in humans. Developing a rapid and low-costdetection method is crucial, especially for developing countries. This study focuses on the development of an efficient loop-mediated isothermal amplification (LAMP) method for accurateidentification. A new LAMP primer set was designed, and a LAMP reaction was optimized. The colorimetric detection method was also applied, and the assay characteristics were evaluated using clinical samples. The results demonstrated a specific LAMP reaction fordetection of genotypes A, B, and C, with a limit of detection of 10cfu/mL, 100% specificity and sensitivity, and a rapid detection time of only 40 min by colorimetric visual inspection. No cross-reactivity with reference strains of,,,, andtype b was observed in the LAMP reaction with the new primer set. This result suggests that the LAMP reaction could be a promising tool for developing a rapiddetection method suitable for use in Vietnam and other developing countries.