miR-1236-3p targets Toll-like receptor 4 to suppress the anti-activity of macrophage.

() modulates host innate immunity via Toll-like receptor 4 (TLR4), associated with the susceptibility to. Bioinformatics predicted miR-1236-3p could be a potential target for the 3'-UTR of thegene. However, the clinical significance and underlying mechanisms remain unclear. To validate this, we analyzed miR-1236-3p levels in 81 subjects and observed that both active tuberculosis (ATB) and latent tuberculosis infection (LTBI) patients exhibited elevated miR-1236-3p levels compared to healthy control (HC) subjects.dual-luciferase reporter assays confirmed that miR-1236-3p specifically targeted the 3'-UTR ofmRNA. Duringinfection in macrophages, miR-1236-3p enhanced the NF-κB signaling and reduced the release of intracellular inflammatory factors, reactive oxygen species, and nitric oxide (NO), indicating that the ability of macrophages to constrain intracellularinfection was compromised by miR-1236-3p. In summary, miR-1236-3p may target/NF-κB signaling to suppress the intrinsic anti-activity of macrophage.