Correlation of (IL-10) gene rs 1800896 polymorphism and tuberculosis risk in Iraq's Nineveh city

The equilibrium between host immune factors and bacterial evasion methods is critical for tuberculosis (TB) control. One immunomodulatory factor that has been suggested to affect a person's vulnerability to tuberculosis is interleukin-10 (IL-10).The study aimed to Identify: (1) Association of (IL-10) gene rs 1800896 polymorphism and the hazard of TB infection, (2) a novel variation of this gene that may be influencing its activity, (3) Association of some haematology tests and tuberculosis. The study included 334 patients with tuberculosis and control. DNA was extracted from blood samples, (IL-10) gene rs 180096 polymorphism was detected by Tetra ARMS PCR and gene sequencing method. (IL-10) levels were detected by Elisa test. Sequencing results for amplifying of the (IL-10) gene in tuberculosis patients revealed the existence of several nucleotide sequence variations that have not been reported before. Both a new phenotype of the (IL-10) protein and a new genotype for the (IL-10) gene were discovered in Nineveh, Iraq, at the National Center for Biotechnology Information. The ARMS PCR reaction results indicated a correlation between the genomic variation of the (IL-10) gene at the site (rs 180096) and tuberculosis patients. The genetic variation was observed in three different genotypes with varying proportions. The native allele C had a higher allelic frequency (65%) than the mutant allele T (35%). The study concluded that individuals with the (IL-10) gene rs180096 TT genotype under the dominant model were more likely to be at risk of TB infection.