Enhancement of M. tuberculosis Line Probe Assay Sensitivity through Whole Genome Amplification of Low-Quantity DNA Released from Sputum and Archived on Chemically-Coated Cellulose Matrix Using an Isothermal Enzymatic Strand-Displacement Process
Ikechukwu Jude Onyia, Uzoamaka Ogechi George-Okafor, Ujunwa Felicia Nwachukwu
International Journal of Biotech Trends and Technology · 2025-03
Abstract
Enhancement of M. tuberculosis Line Probe Assay Sensitivity through Whole Genome Amplification of Low-Quantity DNA Released from Sputum and Archived on Chemically-Coated Cellulose Matrix Using an Isothermal Enzymatic Strand-Displacement Process
MeSH terms
- Loop-mediated isothermal amplification
- Sputum
- Mycobacterium tuberculosis
- DNA
- Multiple displacement amplification
- Tuberculosis
- Cellulose
- Matrix (chemical analysis)
- Materials science
- Molecular biology
- Displacement (psychology)
- Enzyme
- Sensitivity (control systems)
- Chemistry