Synthesis of Hydrazides Derivatives as Anti-Mycobacterial Agents Mediated by the Environmental-Friendly Organocatalyst

Background: Hydrazide derivatives were synthesized using an organocatalyst, offering an environmentally friendly approach characterized by shorter reaction times, mild conditions, and the use of green solvents. Objective: The objective was to evaluate the synthesized compounds for their anti-tubercular ac-tivity against Mycobacterium tuberculosis (Mtb) and to assess the cytotoxicity of active com-pounds using the MTT assay. Methods: Ultrasound energy was employed to facilitate the synthesis of the compounds. The re-sulting compounds were tested for their activity against Mycobacterium tuberculosis (Mtb), and the active compounds were further evaluated for cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl) -2,5- diphenyl tetrazolium bromide MTT assay. Results: Several compounds demonstrated activity against Mtb, with some derivatives exhibiting a Minimum Inhibitory Concentration (MIC) of 1.56 μg/mL, comparable to the standard anti-tu-bercular drug Ethambutol. Conclusion: The eco-friendly synthesis of hydrazide derivatives shows significant potential in the development of anti-tubercular agents, with some compounds displaying efficacy similar to estab-lished drugs.