RNA Extraction and RNA-Sequencing Method for Transcriptomic Analysis of Mycobacterium tuberculosis v1

RNA-sequencing (RNA-seq) technologies have advanced exponentially in recent years, however application of RNA-seq to M. tuberculosis remains limited. We present a complete wet-lab and computational protocol for RNA-seq of Mycobacterium tuberculosis including RNA extraction, rRNA depletion, cDNA library preparation, high-throughput sequencing, and differential expression analysis. This research contributes to the literature by providing methodology for RNA-seq of M. tuberculosis, thereby bridging an important gap in mycobacterial transcriptomics.