A review on Paratuberculosis (John’s disease) current diagnostic approaches and future prospects

The objective of the present study is to review the current diagnostic tools used for detection of paratuberculosis infection in animals. It also showed the sensitivity and specificity of each test as well as the difficulty of fecal culture as gold standard technique for paratuberculosis infection diagnosis. It also reviewed the limitation of commercial Elisa tests as they cannot be used for diagnosis of animals at early stages of infection. Lateral flow test has been recently developed. We suggest it could be used as rapid, accurate useful tool for diagnosis of MAP infection.