P-type ATPase zinc transporter Rv3270 of Mycobacterium tuberculosis enhances multi-drug efflux activity

Graphical abstract : The probable model depicting antibiotic export by Rv3270 (a) Antibiotics are reported to enter the cells through porin channels such as Msp family porins .(b) Zn 2+ (green dots) imported into the cells through probable Zn 2+ importers such as ZIP transporters. (c) Zinc inside the cells may influence the efflux of antibiotics by a co-transportation process through Rv3270 triggered by ATP hydrolysis. (d) Rv3270 possibly captures beta-lactams and extrudes them out of the cells via hypothetical linking proteins (outer membrane efflux adaptor proteins) and porins (Msp family) like AcrAB-TolC and Rv0194. This maintains a lower concentration of the antibiotic inside the cell, reducing its chances of exerting inhibitory effects on cell wall synthesis.