[Characterization of a 3-dimensional tuberculosis spheroid model constructed using human monocytic THP-1 cells and <i>Bacillus Calmette-Guerin</i>].

OBJECTIVE: . METHODS: test kit, and a waterproof pen PH meter were used to detect the differences in the microenvironment between BCG-infected and non-infected 3D tuberculous spheroids. The utility of this 3D tuberculous spheroids for assessing antibiotic effects of rifampicin and levofloxacin was evaluated by plate colony counting. RESULTS: content and nearly a neutral PH. The tuberculous spheroid model was capable of evaluating the efficacy of anti-tuberculosis drugs, and among them rifampicin showed a stronger antibacterial effect. CONCLUSION: The 3-D tuberculous spheroid model established in this study provides a useful platform for studies of tuberculous granuloma.