A brief report on the of mycobacterial proteins regulated by the loss of PtkA in Mtb-H37Ra

Abstract Kinase-phosphatase signaling plays an important role in cellular signaling pathways. The genome of Mycobacterium tuberculosis , (the causative agent of tuberculosis), is embossed with several kinases and phosphatases. Recently identified protein tyrosine kinase (PtkA) is a novel tyrosine kinase that phosphorylates protein tyrosine phosphatase A (PtpA) and regulates its secretion in the cytosol. PtkA is considered as a regulatory protein. In this study, the mycobacterial proteins regulated due to the ptkA gene deletion in Mtb-H37Ra have been studied and highlighted using two-dimensional (2D) gel electrophoresis coupled with MALDI TOF MS/MS analysis. We have identified 13 differentially expressed proteins that play an important role in mycobacterial intracellular survival and pathogenesis.