Study on the effect of γδ T cells expanded <i>in vitro</i> to kill hepatocellular carcinoma cells

Background γδ T cells for tumor cell immunotherapy has recently become a hot topic. Objective To investigate the stimulation of expanded γδ T cells in vitro to kill liver cancer cells and its mechanism, and in vivo validation. Methods Peripheral blood mononuclear cells (PBMCs) were isolated and amplified. The proportion of γδ T cells in T cells was determined using flow cytometry. γδ T cells were selected as effector cells, and HepG2 cells as target cells in the cytotoxicity experiment. NKG2D blocker was used to block effector cells from identifying target cells, and PD98059 was used to block intracellular signaling pathways. The nude mice tumor model was established in two batches, the tumor growth curve was drawn, and the tumor formation effect was tested using small animal imager to verify the killing effect of γδ T cells. Results The γδ T cells in the three experimental groups exhibited a large amount of amplification (P Conclusion ZOL has high amplification efficiency and a positive effect on killing tumor cells.