Evaluation of the Commercial Kit SIRE Nitratase for detecting resistant Mycobacterium tuberculosis in Brazil
Silvana Spíndola de Miranda, Isabela Neves de Almeida, Maria Luiza Diniz de Sousa Lopes, Jamilly dos Reis de Figueiredo, Lida Jouca de Assis Figueredo, Afrânio Lineu Kritski, Wânia da Silva Carvalho, Maria de Fátima Filardi Oliveira Mansur
Figshare · 2022-01
Abstract
Abstract INTRODUCTION: This study aimed to evaluate a new commercial kit, Kit SIRE Nitratase-PlastLabor, for testing the drug susceptibility of clinical Mycobacterium tuberculosis isolates. METHODS: The accuracy of the Kit SIRE Nitratase was evaluated by examining the susceptibility (streptomycin, isoniazid, rifampicin, and ethambutol) of 40 M. tuberculosis isolates, using the proportion method with Lowenstein-Jensen medium or the BACTEC MGIT 960 system. RESULTS: The detection accuracy for streptomycin, isoniazid, rifampicin, and ethambutol was 95%, 97.5%, 100%, and 80%, respectively. CONCLUSIONS: The exceptional accuracy demonstrated by Kit SIRE Nitratase for isoniazid and rifampicin makes the kit an attractive option for screening M. tuberculosis strain resistance.
MeSH terms
- Sire
- Mycobacterium tuberculosis
- Tuberculosis
- Microbiology
- Biology
- Virology
- Veterinary medicine
- Computational biology