Novel Automation of an Enzyme-Linked Immunosorbent Spot Assay Testing Method: Comparable Diagnostic Performance of the T-SPOT. <i>TB</i> Test Using Manual Density Gradient Cell Isolation versus Automated Positive Selection with the T-Cell <i>Select</i> Kit
Shuhua Wang, Murgesean V. S. Rajaram, André Trollip, Qian Wu, Doris Ayala, Danyelle Garza, Marielena A. Benavidez, Kelsie Fox, et al. (18 authors)
Journal of Clinical Microbiology · 2022-08
Abstract
The diagnosis of latent tuberculosis (TB) infection (LTBI) is critical to improve TB treatment and control, and the T-SPOT. TB test is a commercial enzyme-linked immunosorbent spot assay used for this purpose. The objective of the study was to increase automation and extend the time between blood collection and processing for the T-SPOT. TB test from 0 to 8 h to 0 to 54 h.
MeSH terms
- Dried blood spot
- Peripheral blood mononuclear cell
- Medicine
- Confidence interval
- Tuberculosis
- Whole blood
- Immunology