Bacterial co-infection in a patient with COVID-19 pneumonia induce to release TNF-a

The aim of study: to investigate which bacteria are the most common pathogen associated with COVID-19, and to investigate if the TNF-alpha cytokines at gene and protein levels is changed not only with Klebsiella infection but also change with the COVID-19 infection, using real-time qPCR and ELIZA method. Methodology: 145 specimens of sputum and blood were collected from different hospitals in Al-Najaf city to isolate Klebsiella pneumonia associated COVID-19 infection, and only COVID-19 during 4 months from 24st September 2021 to 24th January 2022). 10 ml of fresh venous blood samples were collected from COVID-19 infected patients for serological and immunological assay for measuring the levels of TNF-a at mRNA and protein levels. The results: We report 150 cases of a different-aged who presented with worsening shortness of breath in the context of COVID-19 complicated by Klebsiella pneumoniae var pneumonia. These case serves to highlight the possibility of bacterial superinfections. The present study has tested whether Klebsiella pneumoniae and COVID-19 is colonized in the lower respiratory tract of patients who were admitted in hospital with COVID-19 pneumonia.