Molecular Characterization of Klebsiella Pneumoniae Isolated From Sputum

Abstract Background: K. pneumoniae is a common opportunistic pathogen responsible in clinical practice and an important pathogen of nosocomial infection. This study aimed to analyze the trend of antimicrobial susceptibility and virulent characteristics of K. pneumoniae isolated from sputum in clinics and to provide data for clinical treatment of K. pneumoniae infection disease. Results: The resistance rates of the 20 K. pneumoniae isolates against 13 antibiotics ranged from 15.0% to 80.0%. The detection rate of ESBLs was up to 55%, and bla SHV were the most prevalent ESBLs genes. Four strains (25.0%) of K. pneumoniae presented HMV phenotype, and 18 strains (90.0%) had the stronger biofilm-forming ability. wzi , wab G , fim H , mrk D were the most prevalent virulence genes. 10 strains were present capsule typing and the higher genetic diversity of colonizing K. pneumoniae in this region. K 19 exhibited a strong positive correlation with imipenem resistance, and K 1 showed strong correlations with mag A . HMV phenotype show significantly negative correlations with multidrug-resistant. Conclusions: The antibiotic resistance of K. pneumoniae isolated from sputum in this hospital was serious, and these strains show the higher genetic diversity.