PROTEIN IDENTIFICATION OF AMINOGLYCOSIDES RESISTANT MYCOBACTERIUM TUBERCULOSIS BY MALDI-TOF MS AND BIOINFORMATICS TOOLS

Tuberculosis (TB) is the major cause of death worldwide.The enormity of the problem has been further worsened by the emergence of multi-drug resistant (MDR) strains and the dual infection with HIV & diabetes.A newly identified TB threat which leaves patients virtually untreatable using currently available anti-TB drugs is XDR-TB.Aminoglycosides are commonly used in the tuberculosis treatment and are drug of choice especially for the category II patient.They inhibit protein synthesis in susceptible bacteria by interacting with several steps of the translationGenomic studies are also helpful to understand the resistance mechanisms, but still our knowledge is incomplete.In any cell, proteins are ultimately working moiety, therefore proteomic analysis of mycobacteria in relation to aminoglycosides resistance could be much informative.The aim of the present study was to analyze and compare the lipophilic proteins of total sensitive and aminoglycosides resistant M. tuberculosis clinical isolates.On analyzing 2D gels, six proteins were found to be over expressed and were identified using MALDITOF MS.These proteins were further characterized by bioinformatics tools such as TBPred, DAS, phylodendron, I-tasser, patchdock, firedock etc.