Molecular Detection of bla(SHV), bla(IMP) and bla(VIM) ?-lactamases genes among Klebsiella pneumonia strains

Spreading of antibiotic resistance genes producing bacteria among patients usually caused potentialinfections for human with high morbidity and mortality especially Klebsiella pneumoniae and the fastdevelopment of Multi-drug resistant (MDR) strains has significantly increased and can be quite problematic.Thus, the current study was aimed to determine the prevalence of antibiotic resistance genes (?-lactamase)among K. pneumoniae isolates. Methods: Fifty K. pneumoniae isolates were collected from sputum samplesfor patients suffered respiratory infection. The detection of resistance genes (blaSHV, blaVIM, bla IMP)was performed by polymerase chain reaction (PCR). Results: The frequency of ?-lactamase-positive K.pneumoniae was 28%, and the prevalence of the blaSHV, blaVIM and blaIMP genes was 100%, 0% and 0%respectively. Conclusions: These results ?-lactamase genes believing multi-drug resistance genes. This helpto decrease treatment problems as a result of resistant bacterial infections and prevention of transmission ofdrug resistance by rapid discovery infection-control programs.