Study on the value of molecular biology combined with liquid MGIT culture method in clinical examination of mycobacterium tuberculosis.

OBJECTIVE: To investigate the clinical value of molecular biology (xpert MTB/RIF) combined with a liquid mycobacterium culture tube (MGIT) in the clinical examination of Mycobacterium tuberculosis (MTB). METHODS: A total of 782 patients with suspected pulmonary tuberculosis who came to our hospital from February 2018 to February 2020 were selected as the research subjects. Sputum samples of all patients were taken in the morning, and BACTEC MGIT culture and xpert MTB/RIF, Lowenstein-Jenden (L-J) culture and acid fast staining microscopy were used respectively to detect the sputum samples. The positive samples were cultured, and the bacteria identification and liquid drug sensitivity test were carried out. We also analyzed the value of xpert MTB/RIF combined with MGIT culture method in the clinical testing of MTB. RESULTS: =61.31, 45.33, P<0.001). (2) The average culture time of 13 smear negative xpert MTB/RIF negative specimens was 17.02 days. The average culture time of 82 smear negative xpert MTB/RIF positive samples was 14.12 days. The average culture time of 77 smear positive xpert MTB/RIF positive samples was 7.45 days. (3) The sensitivity of Xpert MTB/RIF test for rifampicin resistance is 100% (11/11), and the specificity is 91.14% (144/158). CONCLUSION: The method of molecular biology combined with MGIT has a high sensitivity and specificity for clinical diagnosis of MTB.