Identification and Characterization of Mycobacterial Species Using Whole-Genome Sequences
Riojas MA, Frank AM, Greenfield SR, King SP, Meehan CJ, Strong M, Wattam AR, Hazbón MH
Methods in molecular biology (Clifton, N.J.) · 2021-01
Abstract
Whole-genome sequencing (WGS) has shown immense value in enabling identification and characterization of bacterial taxa. This is particularly true for mycobacteria, where culture-based characterization becomes delayed by the inherently slow growth rate of these organisms. This chapter reviews the general techniques behind WGS and their optimization, existing techniques for species-level identification and the advantages of WGS for this purpose, and a variety of useful tools for the genomic characterization of mycobacterial strains.
MeSH terms
- Humans
- Mycobacterium tuberculosis
- DNA, Bacterial
- Microbial Sensitivity Tests
- Sequence Analysis, DNA
- Genomics
- Genome, Bacterial
- Whole Genome Sequencing