Study on the relationship between the expression of NFκB1 and LncRNA-PACER in peripheral blood mononuclear cells of patients with pulmonary tuberculosis

Objective: To investigate the expression relationship between nuclear transcription factor
\nkappa B1 (NFκB1) and long non-coding RNA PACER (LncRNA-PACER) in peripheral
\nblood mononuclear cells (PBMCs) of patients with pulmonary tuberculosis. Methods: From
\nFebruary 2018 to March 2019, 40 patients with pulmonary tuberculosis (tuberculosis group)
\nand 40 healthy persons (control group) were collected, the levels of TNF-α, IL-6 and IL-8
\nin serum were detected by enzyme-linked immunosorbent assay (ELISA); the expressions of
\nLncRNA-PACER and NFκB1 mRNAs in PBMCs were detected by real-time fluorescence
\nquantitative PCR; Western blot was used to detect the expressions of NFκB1 and COX 2
\nin PBMCs; Pearson method was used to analyze the expressions of LncRNA-PACER and
\nNFκB1 in PBMCs of patients with pulmonary tuberculosis, and the expressions of LncRNAPACER
\nand NFκB1 in PBMCs of patients with pulmonary tuberculosis were analyzed.
\nResults: Compared with the control group, the expressions of TNF-α, IL-6 and IL-8 in the
\nserum of patients with pulmonary tuberculosis was significantly increased (P<0.05), and the
\nexpressions of LncRNA-PACER, NFκB1 mRNAs, proteins and COX-2 protein in PBMCs
\nwere significantly increased (P<0.05). The expressions of LncRNA-PACER and NFκB1
\nproteins in PBMCs were related to the number of pulmonary lesions and pulmonary cavity
\n(P<0.05), and there was a positive correlation between the expression of LncRNA-PACER
\nand the expression of NFκB1 mRNA in PBMCs of patients with pulmonary tuberculosis (r =
\n0.873, P<0.05). Conclusions: The expressions of NFκB1 and LncRNA-PACER in PBMCs of
\npatients with pulmonary tuberculosis are significantly increased, they are positively correlated
\nand both of them are related to the occurrence and development of pulmonary tuberculosis.