Phenotypic and functional characterization of lung resident lymphocytes of BCG vaccinated mice

Abstract Mycobacterium tuberculosis (Mtb) remains to be a leading cause of morbidity and mortality, causing an estimated 1.3 million deaths annually. It is known that intranasal Mycobacterium bovis Bacille Calmette-Guérin (BCG) vaccination in mice has proven to provide superior protection against pulmonary TB, as compared to parenterally administered BCG. In the current study, we determined the phenotype and function of lung-resident lymphocytes in intranasally BCG vaccinated mice. C57BL/6 mice were vaccinated intranasally with live-attenuated BCG and lung lymphocytes were isolated and stained for immunophenotyping via flow cytometry at 24, 48, and 72 hours post-vaccination. At 48 hours post-vaccination, we have observed an expansion of CD69+, CD103+, and CD69+CD103+ lymphocytes within the lung of vaccinated mice. Studies are underway to determine the phenotype and function of lung-resident lymphocyte subsets and their expansion capacity at three months after BCG vaccination and Mtb infection.