Clinicomicrobiological Profile of Gram Negative Bacterial Infections in Adult ICU’s of a Tertiary Care Centre

Objectives: To identify the common Gram negative bacteria associated with health care associatedinfections,To study the antibiogram and to identify the presence of genes responsible for Carbapenemresistance in Gram negative bacterial pathogensMethod: Prospective study was carried out for 2 years. The pathogens from cases of clinically suspectedinfection in ICU were identified by the standard biochemical reactions. Antibiotic sensitivity testing wasdone by the Modified Kirby-Bauer disk diffusion method . Multiplex PCR was done to detect the carbapenemresistant genes in Gram negative bacilli :blaNDM-1, blaVIM, blaIMP,blaOXA-48 and blaKPC genesResults: Study included 175 clinically significant Gram negative bacterial isolates .42% of the isolateswere from ET aspirate, followed by sputum,pus and blood. Klebsiella spp. and Acinetobacter spp. were thecommon pathogens .18% of the pathogens showed Carbapenem resistance. The statistically significant riskfactors were Invasive procedure,ventilation,catheterization, prior antibiotic use, chronic liver,lung diseasesand diabetes. NDM 1 was the common gene identified.Conclusion: Antibiotic resistance is on rise in our setup mandating continuous monitoring of the trend .