STUDY OF PHENOTYPE AND GENOTYPE DIVERSITY OF BIOFILM FORMATION AND IDENTIFICATION OF 16SRRNA GENE IN MULTIDRUG RESISTANCE ACINETOBACER BAUMANNII ISOLATED FROM DIFFERENT CLINICAL SAMPLES IN BAQUBA/ IRAQ

Acinetobacter baumannii is nosocomial infection and apportunistic pathogens that have emerged as problems hospital pathogens due to it's biofilm formation ability and multiple antibiotic resistance. The current study included collection of one hundred and thirty samples from patients in Baquba Teaching Hospital in Diyala, during the period from July to December 2019 . The sample included urine, sputum, wound swab, burns, and blood. All isolates were identification by using microscopic , biochemical tests and confirmed by VITEK2 compact system . It was found ( 64 ) A.baumannii isolates ( 9.23% ; n = 12 ) of isolates were multidrug resistance . In this MDR A.baumannii were showed high resistance against different types of antibiotics; it was follows : P / T 100% , CAZ . 96.88% . CRO 95.31% , IPM 56.25% , MEM 54.69% , AK 75% , GM 76.56% , LEV 79.69% , CIP 78.19% ; STX 81.25% , TE 3.12% , and colistin 1.56% .The phenotype study of Biofilm formation after calculation the mean of three measurement during three consecutive days by microtiter plate method , show ( 16.67% ; n = 2 ) isolates were cannot produce biofilm , ( 41.66% ; n = 5 ) isolates were weak biofilm ( 25% ; n = 3 ) isolates were moderate biofilm and (16.67% ; n = 2) isolates were strong biofilm . Based on 16SrRNA gene analysis and phylogenetic tree of most clinical isolates ( 3 , 4 , 6 , 7 , 10 , 11 ) shown new sequencing when compared with NCBI . The molecular detection biofilm related genes , it was found ( 100% ) for csuE , bap and ompA genes , ( 8.33% ) for blaPER-1 , while no isolates showed a positive for blaNDM gene , the phylogenetic tree for ( 5 ) isolates A.baumannii sequencing analysis for csuE, bab, ompA gene show matching in the rate of 100% when comparait with NCBI .