Molecular Detection of Enterotoxin Genes sea, seb, sec, sed and see in Staphylococcus aureus Isolated from Different Clinical Sources in Erbil City -

Staphylococcus aureus is important pathogens that can produce wide range of toxins.Enterotoxins are among those staphylococcal virulence factors that can cause infection and diseases to human. S. aureus strains show variability in possessing enterotoxin SEs genes, most strains possess one or more of the SEs genes, while some strains possess none of them. The current study aimed to use PCR technique for detection the presence and prevalence of SEs genes (sea, seb, sec, sed and see) of S. aureus, also the distribution of those genes among different strains. In this study a total of thirty positive isolates collected from patients who were admitted to Rizgary Teaching Hospital during the period from January 2020 to March 2020. Samples collected from different clinical sources: from burns, surgical wounds blood, sputum, and urine samples. Only those isolate included which identified as positive using cultural, microscopical, biochemical tests, and confirmed by VITEK2 compact system. PCR technique was used for the detection of enterotoxin gene sea to see in S. aureus isolates and the results showed that 13 (43.33%), 3 (10%), 16 (53.33%), 20 (66.67%) and 0 (0%) of the isolates were positive for sea, seb, sec, sed and see genes respectively, also results showed variability among strains in having SEs genes.