Genotype of M. tuberculosis in tuberculosis patients registered in megapolis

<b>Background:</b> Migration has a significant impact on the spread of tuberculosis (TB) in Moscow. Cases of TB detected among internal migrants (residents of other Russian entities) and external migrants (citizens of other countries) account for more than 50% of new TB cases. The study includes a retrospective analysis of the M. tuberculosis genotype for various populations, depends on the place of origin. <b>Aim:</b> To characterize the M. tuberculosis genotype among patients from various populations. <b>Methods:</b> Molecular tests of 765 strains collected in 2014-2015 from 765 new TB cases, registered in all 12 Moscow administrative units were considered. The analysis included spoligotyping data of M. tuberculosis and epidemiological data. <b>Results:</b> Among 765 strains the Beijing family amounted to 424 strains (55.4%; 95%CI 51.8-59.0%), T family – 100 (13.1%; 10.8-15.7%), LAM – 80 (10.5%; 95%CI 8.4-12.9%), URAL – 52 (6.8%; 95%CI 5.1-8.8%), Haarlem – 38 (5.0%; 95%CI 3.5-6.8%). A comparison of the genotype family and various populations showed the absence of significant differences. However, the following trend is detected: Haarlem family is more common among residents 5.7% (95%CI 3.9-8.0%) (p= 0.118) and less among internal migrants 2.8% (95%CI 0.8-7.1%) (p= 0.189). Also the share of Beijing family among patients from Middle Asia is bigger – 65.9% (95%CI 44.3-82.8%) comparing to cases from other Russian entities 54.9% (95%CI 46.4%-63.3%). <b>Conclusions:</b> The obtained data will help to evaluate the delivery of TB infection through the migration by assessment of specific M. tuberculosis genotypes in various populations. Continuation of the study, sample increasing and supplementation by prospective analysis are planning.