Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru
Juan A. Flores, Róger Calderón, Annelies W. Mesman, Martín Soto, Julia Coit, Juan Aliaga, Milagros Mendoza, Segundo R. León, et al. (16 authors)
The Pediatric Infectious Disease Journal · 2020-07
Abstract
We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%-42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%-13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%-100%).
MeSH terms
- Buccal swab
- Medicine
- Mycobacterium tuberculosis
- Tuberculosis
- Polymerase chain reaction
- Confidence interval
- Mycobacterium tuberculosis complex
- Buccal administration
- Microbiology