The Effect Of 1,25-dihydroxyvitamin D To The Phagositosis Activity Of Children Macrophage In Tuberculosis Infection

Introduction: Children infected with TB do not always experience TB pain, depending on the number of bacteria that enter and the amount of endurance the child has. 1,25-dihydroxyvitamin D (1,25 (OH) 2D) is a function modulator for macrophages. Objective: This study aims to determine the effect of giving 1,25 (OH) 2D on the phagocytic activity of macrophages in children infected with TB. Methods: This study was an experimental Post Test Only Control Group Design study of 27 samples of children infected with TB. The 1,25 (OH) 2D concentration used was 10-8M and the concentration of 10-7M. Examination of phagocytic activity is carried out using latex beads. Results: One Way ANOVA analysis showed a significant difference in the percentage of macrophages that phagocyte latex and the average number of latex phagocytes by each macrophage between the control group and the treatment group (p <0.05). In the Post Hoc Bonferroni analysis, the mean percentage of macrophages that phagocyted latex was significant in group K with P2 (p = 0.01) and group P1 with P2 (p = 0.03), while the mean percentage of macrophages that phagocyted latex was not significant in group K with group P1 (p = 0.13). The mean number of latex particles phagocyted by each macrophage was significantly found in group K with P1 (p = 0.01) and group K with P2 (p = 0.01), while the mean number of latex particles phagocytized by each macrophage was not significantly found in group P1 with group P2 (p = 0.91).