Asymmetric trehalose analogues to probe disaccharide processing pathways in mycobacteria
Parker HL, Tomás RMF, Furze CM, Guy CS, Fullam E
Organic & biomolecular chemistry · 2020-05
Abstract
The uptake and metabolism of the disaccharide trehalose by Mycobacterium tuberculosis is essential for the virulence of this pathogen. Here we describe the chemoenzymatic synthesis of new azido-functionalised asymmetric trehalose probes that resist degradation by mycobacterial enzymes and are used to probe trehalose processing pathways in mycobacteria.
MeSH terms
- Mycobacterium tuberculosis
- Trehalose
- Disaccharides
- Microscopy, Fluorescence
- Carbohydrate Conformation