Diagnosis of pulmonary tuberculosis from gastric aspirate samples in nonexpectorating pediatric patients in a tertiary care hospital
Sharma S, Shulania A, Achra A, Jeram H, Kansra S, Duggal N
Indian journal of pathology & microbiology · 2020-04
Abstract
Objectives The aim of this study was to assess the utility of Xpert assay, Ziehl-Neelsen (ZN) staining, and Mycobacteria Growth Indicator Tube (MGIT™) culture for diagnosis of pediatric pulmonary tuberculosis from gastric aspirate (GA) samples and to compare Xpert assay and ZN staining with MGIT rapid liquid culture. Materials and methods GA samples from 210 nonexpectorating children, aged between 6 months to 12 years, presenting to the pediatric out-patient department (OPD) with clinical suspicion of tuberculosis (TB) were collected. The samples were tested by GeneXpert, ZN staining, and MGIT liquid culture. Results GeneXpert is a more sensitive method for rapid and early diagnosis of pediatric TB when compared with microscopy.
MeSH terms
- Sputum
- Gastrointestinal Contents
- Humans
- Mycobacterium tuberculosis
- Tuberculosis, Pulmonary
- Colony Count, Microbial
- Sensitivity and Specificity
- Nucleic Acid Amplification Techniques
- Child
- Child, Preschool
- Infant
- Female
- Male
- Tertiary Care Centers