16S Amplicon PCR for the V3-V4 region for the MicroCOPD samples v1

This is the protocol for DNA extraction, PCR, and MiSeq sample preparation for the microbiome samples from sputum, and the bronchoscopies in the Bergen COPD Cohort study, Bergen COPD Exacerbation Study, the Bergen COPD Microbiome Study and the Bergen ILD Microbiome Study. Slightly modified Illumina protocol for our low-biomass airways samples in the Bergen COPD & ILD studies. The protocol that was the template for the PCR and sample preparation is: 16S Metagenomic Sequencing Library Preparation Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System Part # 15044223 Rev.B.