Direct Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis by Use of <i>pncA</i> PCR Sequencing
Kingsley King-Gee Tam, Peter Kam‐Keung Leung, G. G. Siu, Kwok Chiu Chang, Samson S. Y. Wong, Pak‐Leung Ho, Eunice Ka-Chun Leung, W C Yam
Journal of Clinical Microbiology · 2019-06
Abstract
An in-house-developed pncA sequencing assay for analysis of pyrazinamide (PZA) resistance was evaluated using 162 archived Mycobacterium tuberculosis complex (MTBC) isolates with phenotypic PZA susceptibility profiles that were well defined by analysis of Bactec MGIT 960 PZA kit and PZase activity data. Preliminary results showed 100% concordance between pncA sequencing and phenotypic PZA drug susceptibility test (DST) results among archived isolates.
MeSH terms
- Pyrazinamide
- Mycobacterium tuberculosis
- Microbiology
- Tuberculosis
- Drug resistance
- Biology
- Mycobacterium tuberculosis complex
- Concordance