Modulatory Effects of Verapamil in Rifampicin Activity Against <i>Mycobacterium Tuberculosis</i>
Katiany Rizzieri Caleffi-Ferracioli, Rosilene Fressatti Cardoso, João Vítor Perez de Souza, Letícia Sayuri Murase, Pedro Henrique Canezin, Regiane Bertin de Lima Scodro, Vera LD Siqueira, Fernando R. Pavan
Future Microbiology · 2019-01
Abstract
AIM: To evaluate modulatory effect of verapamil (VP) in rifampicin (RIF) activity and its effect in efflux pumps (EPs) transcript levels in Mycobacterium tuberculosis. MATERIALS & METHODS: RIF and VP minimal inhibitory concentration, combinatory effect and detection of mutations were determined in 16 isolates. EPs transcript levels were determined in four isolates by real-time PCR after exposure to drugs. RESULTS: VP showed good combinatory effect among RIF-resistant isolates. This effect was also observed in the relative transcript levels of EPs, mainly after 72 h of exposure, depending on the EP gene, genotype and the resistance profile of the isolate. CONCLUSION: Additional regulatory mechanisms in the EP activities, as well as, interactions with other drug-specific resistance mechanisms need further investigation in M. tuberculosis.
MeSH terms
- Rifampicin
- Efflux
- Verapamil
- Mycobacterium tuberculosis
- Microbiology
- Tuberculosis
- Biology
- Antibiotics
- Minimum inhibitory concentration
- Drug resistance
- Pharmacology
- Chemistry