NTF-RINT, a new method for the epidemiological surveillance of MDR Mycobacterium tuberculosis L2/Beijing strains
Klotoe BJ, Kurepina N, Zholdibayeva E, Panaiotov S, Kreiswirth BN, Anthony R, Sola C, Refrégier G
Tuberculosis (Edinburgh, Scotland) · 2019-12
Abstract
The most widely discussed antibiotic-resistant tuberculosis strains ("W" and "B0/W148", "CAO") belong to L2/Beijing Lineage and are characterized by IS6110 insertion sequences at the NTF locus. We present a high-throughput, microbead-based method, called NTF-RINT for detection of IS in NTF and Rifampicin and Isoniazid Typing. This method provides tuberculosis diagnostic confirmation, screens for the so-called modern L2/Beijing sublineage and detects mutations involved in resistance to Rifampicin (RIF) and Isoniazid (INH).
MeSH terms
- Humans
- Mycobacterium tuberculosis
- Tuberculosis, Multidrug-Resistant
- DNA, Bacterial
- DNA Transposable Elements
- Bacteriological Techniques
- Population Surveillance
- Reproducibility of Results
- Predictive Value of Tests
- Polymerase Chain Reaction
- DNA Mutational Analysis
- Drug Resistance, Multiple, Bacterial
- Virulence
- Genotype
- Phenotype
- Mutation
- New York City
- Kazakhstan
- Molecular Epidemiology
- High-Throughput Nucleotide Sequencing