Genome Mining of Streptomyces atratus SCSIO ZH16: Discovery of Atratumycin and Identification of Its Biosynthetic Gene Cluster
Sun C, Yang Z, Zhang C, Liu Z, He J, Liu Q, Zhang T, Ju J, et al. (9 authors)
Organic letters · 2019-02
Abstract
Genome mining of the deep sea-derived Streptomyces atratus SCSIO ZH16 enabled the activation of a cyclodepsipeptide gene cluster and isolation of its cinnamic acid-bearing product, atratumycin (1). Atratumycin's structure was elucidated on the basis of extensive spectroscopic experiments, X-ray data, and Marfey's method; a plausible biosynthesis and tailoring modification of 1 are also proposed and investigated. Additionally, atratumycin is active against Mycobacteria tuberculosis H37Ra and H37Rv with MICs of 3.8 and 14.6 μM, respectively.
MeSH terms
- Streptomyces
- Mycobacterium tuberculosis
- Peptides, Cyclic
- Microbial Sensitivity Tests
- Molecular Structure
- Multigene Family