Use of Liquid Chromatography-Mass Spectrometry-Based Metabolomics to Identify Biomarkers of Tuberculosis
Zhou J, Yin Y
Methods in molecular biology (Clifton, N.J.) · 2019-01
Abstract
Liquid chromatography-mass spectrometry (LC-MS) based metabolomics has proven to be a powerful analytical tool for biomarker screening. Here we describe two workflows which employ untargeted metabolomics to study serum biomarkers in tuberculosis patients. Expression profiles for samples of hydrophilic metabolites and hydrophobic metabolites (lipids) may be obtained by this method.
MeSH terms
- Humans
- Mycobacterium tuberculosis
- Tuberculosis
- Lipids
- Chromatography, High Pressure Liquid
- Tandem Mass Spectrometry
- Metabolomics
- Hydrophobic and Hydrophilic Interactions
- Biomarkers