Two new bioactive triterpenoids from the roots of <i>Colubrina asiatica</i>

Two new ceanothane triterpenes, 3,7- O , O -dibenzoyl ceanothic acid methylester ( 1 ) and 3- O -acetyl-7- O -benzoyl ceanothic acid methylester ( 2 ), along with nine known compounds ( 3 - 11 ), were isolated from the roots of Colubrina asiatica . The isolated compounds were identified by spectroscopic evidence. Compounds 1 and 2 showed antimalarial activity against Plasmodium falciparum with IC 50 values of 4.67 and 3.07 µg/mL, respectively. Compound 2 also showed antimycobacterial activity against Mycobacterium tuberculosis (MIC 6.25 µg/mL). In addition, compounds 1 , 2 , 10 and 11 showed cytotoxicity against three cancer cell lines (KB, NCI-H187 and MCF-7) with IC 50 values ranging from 8.32 to 46.72 µg/mL.