<i>Mycobacterium tuberculosis</i> Drug Resistance and Transmission among Human Immunodeficiency Virus-Infected Patients in Ho Chi Minh City, Vietnam
Mai TQ, Martinez E, Menon R, Van Anh NT, Hien NT, Marais BJ, Sintchenko V
The American journal of tropical medicine and hygiene · 2018-12
Abstract
Vietnam has a high burden of tuberculosis (TB) and multidrug-resistant (MDR) TB, but drug resistance patterns and TB transmission dynamics among TB/human immunodeficiency virus (HIV) coinfected patients are not well described. We characterized 200 Mycobacterium tuberculosis isolates from TB/HIV coinfected patients diagnosed at the main TB referral hospital in Ho Chi Minh City, Vietnam. Phenotypic drug susceptibility testing (DST) for first-line drugs, spoligotyping, and 24-locus mycobacterial interspersed repetitive unit (MIRU-24) analysis was performed on all isolates. The 24-locus mycobacterial interspersed repetitive unit clusters and MDR isolates were subjected to whole genome sequencing (WGS). Most of the TB/HIV coinfected patients were young (162/174; 93.1% aged M. tuberculosis transmission among HIV-infected patients.
MeSH terms
- Humans
- Mycobacterium tuberculosis
- HIV
- Tuberculosis, Multidrug-Resistant
- HIV Infections
- Antitubercular Agents
- Retrospective Studies
- Phylogeny
- Drug Resistance, Multiple, Bacterial
- Minisatellite Repeats
- Genotype
- Genome, Bacterial
- Multigene Family
- Adolescent
- Adult
- Middle Aged
- Vietnam
- Female
- Male
- Coinfection
- Whole Genome Sequencing