Assay for improved detection of antigen-specific immune cells from extrasanguinous fluids
Schub D, Fousse M, Elsäßer J, Faßbender K, Sester U, Schmidt T, Sester M
European journal of immunology · 2018-04
Abstract
In this approach, pre-stained cells from extrasanguinous fluids (ESFs) are stimulated in the presence of blood from the same individual. Thus, blood-derived antigen-presenting cells enable stimulation of both ESF- and blood T cells. Pre-staining allows distinction of T cells from ESF and blood, and simultaneous analysis of antigen-specific T cells in both compartments.
MeSH terms
- Antigen-Presenting Cells
- T-Lymphocytes
- Cerebrospinal Fluid
- Humans
- Mycobacterium tuberculosis
- Herpesvirus 3, Human
- Tuberculosis, Pulmonary
- Meningitis, Viral
- Neuritis
- Recurrence
- Antigens
- Immunoassay
- Adult