A Method to Evaluate Persistent Mycobacterium tuberculosis In Vitro and in the Cornell Mouse Model of Tuberculosis
Hu Y, Coates A
Methods in molecular biology (Clifton, N.J.) · 2018-01
Abstract
Persistent Mycobacterium tuberculosis will not grow on solid or liquid media. They will, however, grow in the presence of resuscitation promoting factors (RPF). Here we describe the production of RPF rich culture supernatants, and their use for the stimulation of growth of persisters in vitro as well as in the Cornell model of tuberculosis.
MeSH terms
- Animals
- Mice
- Mycobacterium tuberculosis
- Tuberculosis
- Disease Models, Animal
- Antitubercular Agents
- Female
- Host-Pathogen Interactions
- Bacterial Load
- Hypoxia